Curcumin-loaded niosome formulation with high incorporation efficiency and anticancer activity towards human ovarian cancer cell line
Ashraf Alemi,
1,* Javad zavvar reza,
2 Mohammad farrokhifar,
3 Majid farrokhifar,
4 Zakieh entezari nasab,
5 Ali farrokhifar,
6
1. Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
2. Department of Clinical Biochemistry, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
3. Kar Higher Education Institute of Rafsanjan, Rafsanjan, Iran.
4. Department of Pediatrics, Sabzevar University of Medical Sciences, Sabzevar, Iran
5. Department of Pediatrics, Semnan University of Medical Sciences, Semnan, Iran
6. Faculty of Medicine, Aja University of Medical Sciences, Tehran, Iran
Abstract
Introduction
Ovarian cancer is a common malignant tumor of the female genital organs, and has the highest mortality rate among all gynecological types of cancer. as a potential anticancer agent, curcumin (cur) has been applied for its chemotherapeutic activity in various cancers. however, the clinical application of cur is limited due to poor stability, low solubility, and rapid degradation in vivo. to overcome these issues, a novel curcumin-loaded niosome formulation composed of nonionic surfactants: tween 60 and cholesterol was prepared. aim of this study is use of nanoniosomal containing curcumin and cytotoxic effects on ovarian cancer cell line.
Methods
In this experimental study, curcumin-loaded niosome was formulated by the thin film hydration method. dynamic light scattering (dls) and uv-v spectrophotometry were utilized to investigate the quality of the nanoniosomes. cholesterol and tween 60 were obtained from lipoid gmbh. in addition, mtt assay were used to determine anticancer activity of nanocarriers towards ovcar-3 cell line
Results
Curcumin in the niosomal formulation was encapsulated with a high entrapment efficiency of 98.4%. the average diameter, polydispersity index and zeta potentials of niosome prepared were determined to be 127.5 nm, 0.247 and -6, respectively. the optimized formula exhibited significantly greater cytotoxic activity with ovcar-3 ovarian cancer cell line in comparison free curcumin.
Conclusion
Based on these results, the prepared curcumin niosome demonstrated to be promising as a nano-size carier for the delivery of curcumin and ovarian cancer therapy.
Keywords
Niosome, curcumin, ovarian cancer
