1. Department of Microbiology – shahr-e-Qods Branch – Islamic Azad University –Tehran / Iran 2. Department of Microbiology – shahr-e-Qods Branch – Islamic Azad University –Iranian Gene Fanavar Institute (IGF), Tehran 3. Department of Microbiology – shahr-e-Qods Branch – Islamic Azad University –Tehran / Iran
Abstract
Introduction
Staphylococcus aureus has become the most common cause of hospital acquired infections over the last few decades. the bacterium is a normal microbial flora of the human body, especially in the microbial flora of the skin. nonetheless, immunocompromised patients are at risk of developing infection with this bacterium and the contamination resulting from this pathogen in most cases is also the origin of the hospital. one of the factors contributing to the creation of resistance to mupirocin in staphylococcus aureus, the presence of the mupa gene is that causing for antibiotic resistance to mupirocin. this antibiotic is used to treat staphylococcus aureus skin infections.the aim of this study was to determine the rapid molecular detection of mupirocin resistant staphylococcus aureus isolated from the two hospitals.
Methods
In this study, 100 positive strains of staphylococcus aureus were collected in the muller hinton broth medium from milad and moheb hospitals of tehran. dna of samples were extracted by dng-plus method. pcr test optimized for mupirosin resistance detection and the sensitivity of test evaluated and the samples were tested along with positive and negative controls.
Results
The pcr product of 457 bp was amplified and observed with 1.5% agarose gel electrophoresis. the results show that, 7 samples (7%) has been contained the gene for resistance to mupirocin. the detection limit of this test was measured 500 dna/reaction and in terms of characteristics, the specificity of 100% of primers was shown.
Conclusion
This study showed that molecular methods based on amplification, such as pcr, fast, sensitive and accurate methods to identify antibiotic resistance, especially in staphylococcus aureus
Keywords
Mupirocin, staphylococcus aureus, pcr, drug resistance.
