Effect of crocin on mitochondrial biogenesis and oxidative stress in the striatum of cholestatic rats

Mohammadreza Eteqadi,1,* Mohammad nasehi,2

1. Department of Biology, Faculty of science,Islamic Azad Unversity, Central Tehran branch.Tehran.Iran
2. Department of Biology, Faculty of modern medical science,Islamic Azad Unversity, Medical Tehran branch.Tehran.Iran



Oxidative stress and increment of mitochondrial reactive oxygen species (ros) production that occurs in cholestasis which communicates oxidative stress to development of neurological diseases. cholestasis effects on striatum and striatum also is center of movement learning in the mind. pgc-1α is the master regulator of mitochondrial biogenesis. in the present study we investigate the protective impact of crocin against defects in striatum due to cholestasis in male wistar rat.


Adult rats weighing 200- 250g were randomly divided into five groups including: group 1: normal-control (no operation). group 2: sham-control (underwent laparotomy without bile duct ligation). group 3: bdl- control (underwent laparotomy with bile duct ligation) and all of three groups have not been received drug. group 4: sham- crocin group 5: bdl- crocin were treated with crocin. rats were injected with a daily dose of crocin (30 mg/kg ip) for 30 days, striatum homogenates were obtained for real time pcr examination.


Our results demonstrate that the mean change in pgc-1α expression of bdl- crocin group there is a significant difference (p<0.05) as compared with bdl- control.


The present findings provide evidence that crocin by blockade of ros generation and biogenesis improved may have beneficial effects in the mitochondrial dysfunction and a therapeutic strategy of neurodegenerative diseases such as alzheimer and parkinson diseases.


Crocin-striatum-cholestasis-oxidative stress- mitochondrial biogenesis