Temperature sensitive smart nanocarriers: a novel approach in cancer gene therapy

Noorossadat Seyyedi,1,* Mohammad ali hoshyar,2 Ali farhadi,3 Gholam reza rafiei dehbidi,4 Negin nikouyan,5 Abbas behzad behbahani,6

1. Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences
2. Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences
3. Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences
4. Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences
5. Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences
6. Diagnostic Laboratory Sciences and Technology Research Center, Shiraz University of Medical Sciences

Abstract

Introduction

Gene therapy for cancer treatment still is a major challenge in clinical research. among several types of cancers, malignant solid tumors such as colon, breast, lung, and prostate cancer are clinically important therefore, the best candidates for treatment with gene therapy. various viral and non-viral vectors can be used for gene therapy. however, non-viral vectors for gene therapy present several advantages over viral vectors. among them, nanoparticles play an important role in the target-specific delivery of genes in the body. there are three different methods for gene delivery using nanoparticles including encapsulated inside nanoparticles, forming complexes through ionic interactions with nanoparticles, and loaded on surface via conjugation or modified-polymer trapping.

Methods

Ph- and temperature-sensitive hydrogel nanoparticles (tshnp), a kind of stimuli-responsive nanocarriers, that respond to various internal or external stimuli are investigated for local hyperthermia in a solid tumor as a good endogenous stimulus to activate tshsp and resulting in the release of synthetic cargo.

Results

The results of this study indicated that having a high concentration in the blood, intelligent nanoparticles can be used as a therapeutic solution in specific cases, in particular solid tumor cells due to the angiogenesis with higher temperature than other tissues.

Conclusion

This study provides a comprehensive analysis of progress in novel approaches in cancer gene therapy using temperature sensitive nanocarriers.

Keywords

Polymers; temperature; nanoparticles; genetic therapy; hyperthermia