• Synthesis of MIL-100 nanocarrier and loading of oxaliplatin anticancer drug on it
  • Ensieh sadat Seyed Hossien,1,* Maryam BIKHOF,2 Mitra Salehi,3 Mohammad Yousefi,4
    1. Department of biology - faculty of biological science -North Tehran Branch-Islamic Azad university- Tehran- Iran
    2. Department of biology - faculty of biological science -North Tehran Branch-Islamic Azad university- Tehran- Iran
    3. Department of biology - faculty of biological science -North Tehran Branch-Islamic Azad university- Tehran- Iran
    4. Department of Biology, Yadegar-e-Imam Khomeini (RAH) Shahr-e-Rey Branch, Islamic Azad University, Tehran, Iran


  • Introduction: Introduction: Today, cancer is the second leading cause of death in the world. Despite the great advances that have been made in chemotherapy, researchers are still looking for ways to deliver more effective drugs to the tumor that reduce side effects by slowly releasing the drug. Oxaliplatin is a type of chemotherapy drug used to treat colorectal cancer, which forms an intracellular link between DNA strands that inhibits transcription, disrupts the cell cycle, and ultimately causes apoptosis and cell death. In this study, in order to release oxaliplatin in a controlled manner and increase the effectiveness of chemotherapy, the MIL-100 nanocarrier was introduced as a new class of porous, high-surface crystalline MOFs containing iron oxide (III) clusters are synthesized and after loading the drug on it, the percentage of loading was calculated.
  • Methods: Methods: In this study, first the synthesis of MIL-100 (Fe) was performed by hydrothermal method. The synthesis method is as follows: H2BTC (1.6235g) and FeCl3.6H2O (0.8403g) are dissolved in 30ml of distilled water, then HF (0.213ml) and HNO3 (0.163ml) are added after 30 minutes in the oven for 20 hours at 150 ° C, the precipitate is centrifuged for 4 minutes at 4000 rpm and washed several times with distilled water and ethanol. Then MIL-100 (Fe) was dried in an oven at 150 ° C for 6 hours, and finally the orange powder of MIL-100(Fe) was obtained. The resulting MIL-100 (Fe) sample was confirmed by FTIR and XRD spectroscopy. Then, the drug was loaded on the nanocarrier with a weight ratio of 0.1 to 1 in the PBS buffer by a homogenizer at room temperature and the loading percentage was calculated after drawing the standard oxaliplatin curve.
  • Results: Results: The interpretation of the FTIR spectrum confirmed the synthesis of MIL-100(Fe). As the peak in region 3420 is the tensile vibration related to OH, the peaks of 1590 and 1390 show asymmetric and symmetrical vibrations of the C-O band in the carboxyl group of the trimic acid bonding agent. The peak 750 of the CH-benzene extinction vibration of the benzene ring in trimic acid and the peak of 550 indicate FEO. The XRD spectrum also shows the two main peaks 2 ϑ=4 and 2 ϑ =11 and confirmed the crystallinity of the synthesized nanocarrier. The results of UV absorption spectroscopy showed lambda max value of oxaliplatin at 257nm. Also, oxaliplatin loading percentage on MIL-100 was calculated 52%.
  • Conclusion: Conclusion: The results of the present study showed that MIL-100(Fe) nanoparticles can be used as an efficient drug carrier for loading and delivery of oxaliplatin to cancer cells.
  • Keywords: Keywords: MOF, MIL-100, oxaliplatin, drug delivery