Provide immunity protection of avian immunoglobulin (IgY) against the recombinant VacJ protein of Acinetobacter baumannii in a model of mouse pneumonia
Provide immunity protection of avian immunoglobulin (IgY) against the recombinant VacJ protein of Acinetobacter baumannii in a model of mouse pneumonia
Introduction: Acinetobacter baumannii is one of the pathogens in clinical settings that can cause a variety of infections. The lack of effective antibiotics is convincing researchers to consider new treatment options. Many bacterial lipoproteins are involved in escape from the host immune system, and antibiotic resistance. Therefore, lipoproteins are good options for inducing immunity against many infectious diseases. The VacJ gene is highly conserved and produces an outer membrane lipoprotein that is associated with virulence in many pathogens. as a result, VacJ as a membrane lipoprotein is a suitable target for vaccine design and production.After immunization of laying hens by recombinant proteins (VacJ).
Methods: IgY was purified from the egg yolks.Then, ELISA immunoassays were performed and the protective effect of antibodies was evaluated in a mouse model of A. baumannii pneumonia
Results: Injection of VacJ antigen stimulated the immune system of chickens well. After purification and collection of chicken antibodies to evaluate the protective effect of this antibody, nasal form was given to mice with weakened immune system and then mice were infected with Acinetobacter baumannii. Infected, survival and cultured lung and spleen were assessed. There was a significant difference between the survival of immunocompromised mice with other groups.
Conclusion: Partial protection of avian antibodies against VacJ resulted in 100% survival of the group receiving anti-VacJ antibodies. Also, in organ culture, a significant reduction in bacterial load was observed in the group receiving safe antibodies. Our results showed that VacJ antigen is effective in prevention and is a good candidate for vaccine design.