Redroot pigweed Ethanolic Extract Toxicity on HDFa cells
Redroot pigweed Ethanolic Extract Toxicity on HDFa cells
Hamideh Bakhshayeshan-Agdam,1,*Nastaran Sedghi ,2Seyed Yahya Salehi-Lisar ,3Majid Mahdavi ,4
1. Department of Plant Sciences, Faculty of Natural Sciences, University of Tabriz, Tabriz-5166616471, Iran 2. Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz, Tabriz-5166616471, Iran 3. Department of Plant Sciences, Faculty of Natural Sciences, University of Tabriz, Tabriz-5166616471, Iran 4. Department of Animal Sciences, Faculty of Natural Sciences, University of Tabriz, Tabriz-5166616471, Iran
Introduction: Cancer is currently one of the major causes of mortality and morbidity among humans. Extracts of plants have a potential source for treatments of Cancers, but the extracts toxicity on normal cells is a topic that today is receiving a lot of attention. Redroot pigweed (Amaranthus retroflexus L.) is a weed plant with well-known allelopathic effects. This plant has diverse therapeutic effects such as anti-leukemia properties due to specific biochemicals which involving in its allelopathic interaction as well. Results from MTT assay revealed that redroot pigweed ethanolic extract inhibits the viability and proliferation of NB4 cells, in a time- and dose-dependent manner. So that, 70% decline in viability of treated cells with 400 µg/ml of extract was observed after 72h.
Methods: In order to evaluate redroot pigweed ethanolic extract toxicity on normal cells (HDFa), normal cells treated with lower concentrations than IC50 for NB4 cells (160 µg/ml).
Results: Results from MTT assay showed that redroot pigweed ethanolic extract inhibits the viability and proliferation of HDFa cells in a time- and dose-dependent manner. At highest concentration of amaranth ethanolic extract (160 µg/ml), the viability percentage was more than 60%. Cell's viability in the culture media is declined over time due to influence various factors in the synthetic media, the same situation exists for HDFa cells. Hence, evaluation of apoptotic cells percentage using flowcytometry can provide reliable results for amaranth cytotoxicity.
Conclusion: Overally, this study demonstrates the potential of redroot pigweed ethanolic extract application as an anti-cancer drug for leukemia cancer treatment. Indeed, this investigation showed that the toxicity of the extract on normal cells is such that it could be used for safe treatment.