Comparison the different immunogenicity methods of experimental Neospora caninum vaccine in mice
Comparison the different immunogenicity methods of experimental Neospora caninum vaccine in mice
zahra zarenejad,1Mohammad mehdi Namavari,2,*Ahad olyaei,3Fatemeh Dabiri,4
1. Department of Veterinary Medicine, Kazerun Branch, Azad University, Iran 2. Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran 3. Department of Veterinary Medicine, Kazerun Branch, Azad University, Iran 4. Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran
Introduction: Neospora caninum is a pathogenic coccidial intracellular protozoan of the sarcocyste family that is very common in dairy and beef cattle and can lead to abortion or birth of an infected calf. Neospora caninum is a major economic challenge in the field of husbandry animal around worldwide and can cause abortion, stillbirth, calf mortality, poor calf production, re-breeding and reduced milk production. One of the most effective ways to control infection is to use vaccination. Neospora caninum live-attenuated strain with adjuvant is able to create good immunity in animals. In this study, we evaluated and compared the best immunogenicity method with experimental vaccine of Neospora caninum with adjuvant in BALB / C mice as the best laboratory model for neosporosis.
Methods: Thirty mice were randomly divided into six equal groups, including the control groups, live-attenuated strain with aluminum hydroxide adjuvant, live-attenuated strain with combined adjuvant separately groups by intramuscular injection, subcutaneous injection, intraperitoneal injection and orally; all were immunized in two phases with four weeks apart. Agglutination and ELISA tests were used to assess the humoral immune response and gamma interferon test was used to measure cellular immunity.
Results: The results showed that the immunized group with live-attenuated strain with combined adjuvant by subcutaneous injection had the highest humoral immune response and the immunized group with live-attenuated strain with combined adjuvant by intraperitoneal injection and also by intramuscular injection had the highest cellular immune response.
Conclusion: Overall, the results of this study showed that the selection of an appropriate method for vaccination is effective on humoral and cellular immunity. The live-attenuated vaccine of Neospora caninum with combined adjuvant in mice in different ways is induce an effective humoral and cellular immune response and immunizing mice with this method is safe and completely effective. Therefore, it is suggested that this experimental vaccine and intramuscular or subcutaneous injection method be used in additional research in order to obtain a functional vaccine used to control the infection caused by Neospora caninum in farm animals such as cattle and sheep.