Evaluation the immunity of Neospora caninum attenuated strain in preventing oocyst shedding in dogs
Evaluation the immunity of Neospora caninum attenuated strain in preventing oocyst shedding in dogs
Elham Ghazanfari,1Ahad Olyaei,2Mohammad mehdi Namavari,3,*zahra khabazan,4
1. Department of Veterinary Medicine, Kazerun Branch, Azad University, Iran 2. Department of Veterinary Medicine, Kazerun Branch, Azad University, Iran 3. Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran 4. Shiraz Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Shiraz, Iran
Introduction: The protozoan Neospora caninum is known around the world as one of the most important causes of disaster abortion in cattles, especially when the dogs live as the main host in their surroundings. Currently, the focus is on live vaccines or attenuated vaccines. In this research; Neuspora caninum has been evaluated as an effective vaccine to prevent oocyst shedding in dogs.
Methods: Sixteen dogs were randomly divided into four equal groups, including the live-attenuated strain without adjuvant, acute strain, live-attenuated strain with oily adjuvant, and the control group. All the groups were immunized in two phases, four weeks apart. One month after the second injection, all dogs were challenged by acute NC-1 strain. Agglutination test was used to assess the immune response and molecular test was used to assess oocyte shedding.
Results: The results showed that the immunized group with live-attenuated strain with adjuvant produces the highest immune response. PCR test also showed that dogs immunized with live-attenuated strain with adjuvant did not shed oocyst after challenge. Overall, the results of this study showed that Neuspora caninum live-attenuated vaccine with oily adjuvant in dogs is effective in creating an immune response and controlling oocyst shedding, and immunizing with this method is safe and completely effective.
Conclusion: Therefore, it is suggested that this experimental vaccine will be used in supplementary research in order to obtain a functional vaccine to control the infection caused by Neospora caninum in dogs.