The Antinociceptive Effect of Isoniazid in the Acetic Acid Writhing Test in Mice
The Antinociceptive Effect of Isoniazid in the Acetic Acid Writhing Test in Mice
Amir Abbas Barzegari,1,*Maryam Azaddar,2Babak Vahdani,3Saeed Khalili,4
1. Assistant Professor of Animal physiology, Department of Biology, Faculty of Basics Sciences, University of Maragheh, Maragheh, Iran. 2. Instructor of Animal physiology, Department of Biology, Faculty of Basics Sciences, University of Maragheh, Maragheh, Iran. 3. Master of Science (MSc) student in Microbial Biotechnology, Department of Biology, Faculty of Basics Sciences, University of Maragheh, Maragheh, Iran. 4. Master of Science (MSc) student in Microbial Biotechnology, Department of Biology, Faculty of Basics Sciences, University of Maragheh, Maragheh, Iran.
Introduction: Many neurotransmitters in the central nervous system are involved in pain perception and modulation. Isoniazid, as an important anti-tuberculosis drug, is also a modulator of the GABAergic system in the CNS. As a result, the purpose of the present research was to evaluate the effects of isoniazid on the acetic acid writhing test (a non-selective animal model of pain) in mice.
Methods: Four groups of mice (n=8) were used in this study. In this study, the pain was induced by intraperitoneal injection of acetic acid (0.6%, 10 ml/kg) in mice. Saline or isoniazid at doses 25, 50, and 75 mg/kg was administered intraperitoneally 60 min before acetic acid administration. Then, the abdominal writhes were counted during a 30-min period of the test.
Results: Isoniazid in doses 25, 50, and 75 mg/kg decreased the number of writhes in the experimental groups compared with the saline-treated group. The dose of 75 mg/kg was the most effective dose of isoniazid in pain reduction.
Conclusion: In conclusion, isoniazid has an antinociceptive effect in the writhing test in mice.