Effects of Niosome Nanoparticle Containing Boswellic Acid on the Glioblastoma Cell Proliferation
Effects of Niosome Nanoparticle Containing Boswellic Acid on the Glioblastoma Cell Proliferation
Hamid Reza Moradi,1Gholamhossein Tondro,2Soghra Gholami,3Sajad Sahabnegah,4Ghadir Rajabzadeh,5Zabihollah Khaksar,6,*
1. Department of Basic sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran 2. Department of pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran 3. Department of Basic sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran 4. Department of Neuroscience, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 5. Department of Food Nanotechnology, Research Institute of Food Science and Technology, Mashhad, Iran 6. Department of Basic sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
Introduction: The application of boswellic acid-loaded niosome nanoparticle (BA-NP) is a novel method that can promote the bioavailability and drug delivery activity of free boswellic acid. Human glioblastoma is the most prevalent primary brain tumors that has a higher mortality rate among human cancers. The purpose of this study was the effects of the BA-NP on glioblastoma cell line (GCL) proliferation through MTT assay.
Methods: Glioblastoma cell line (1×104/well) were cultured in 96-well plates and incubated at 37 °C in an atmosphere of 5% CO2 overnight. These cells were incubated with various concentration of BA-NP for the next 24, 48 and 72 h. Then, 10 μl of the MTT solution in PBS (5 mg/ml) was added to each well at a final concentration of 0.05%. After 4 h, the supernatant was removed and to dissolve the formazan crystals, 100 μl of dimethyl sulfoxide was added to each well. Then, the microplates kept in the dark (60 min) and absorbance was measured by an ELISA microplate reader at 550 nm.
Results: Our results showed that BA-NP significantly inhibited the proliferation of glioblastoma cell line in a time- and dose-dependent manner. The IC50 value of BA-NP on GSCs was 0.12 μg/ml after 24 h, which decreased to 0.037μg/ml after 48 h.
Conclusion: In conclusion, results of the current study shown that boswellic acid-loaded niosome nanoparticle have the effects of cytotoxicity and inhibition of proliferation on glioblastoma cell line (U87) in a time- and dose-dependent manner.