Bioinformatics Analysis of the miRNAs, Targeting RUNX3 Gene in Gastric Cancer
Bioinformatics Analysis of the miRNAs, Targeting RUNX3 Gene in Gastric Cancer
Seyed Taleb Houseini,1,*Roya Bishe Kolai ,2Arash Sattari ,3Farkhondeh Nemati ,4
1. Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran 2. Department of Biology, Faculty of Basic Sciences, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Iran 3. Department of Biology , Faculty of Basic Sciences , Islamic Azad University , Gorgan Branch , Golestan , Iran 4. Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Qaemshahr Branch, Mazandaran,Iran
Introduction: Gastric cancer is the fourth common cancer in the world, and the second leading cause of death, due to cancer. It is known that gastric cancer can occur as a result of genetic and epigenetic alterations of the tumor-suppressing genes, regenerating genes, and adhesive molecules. As the miRNAs play a significant role in the regulation of the cellular procedures, this study aims to predict the miRNAs, targeting the RUNX3 gene, using bioinformatics tools.
Methods: After getting the RUNX3 protein chain from the NCBI database, the miRNAs targeting the gene were predicted using TargetScan, miRDB, and miRWalk databases using different algorithms.
Results: Based on the scoring system of the bioinformatics programs and considering the best targeting scores, hsa-miR-106b-5p , hsa-miR-130b-3p , has-miR-454-3p , has-miR-3666 , has-miR-4295 , has-miR-301b-3p were identified as miRNAs targeting RUNX3 gene . They were suggested for the practical studies in future.
Conclusion: Results from bioinformatics studies revealed that of the six miRNAs identified and are probably the targeting miRNAs specific for the RUNX3 gene , respectively . therefore it seems that these miRNAs can be a suitable candidate for in vitro studies in GC patients.