Frequency of genes involved in irp-1 and irp2 iron acquisition in clinical isolates of Klebsiella pneumoniae
Frequency of genes involved in irp-1 and irp2 iron acquisition in clinical isolates of Klebsiella pneumoniae
Fatemeh Bahrami chegeni,1,*
1. Razi Herbal Medicine Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Introduction: Klebsiella pneumoniae is an opportunistic pathogen of Enterobacteriaceae family that causes sepsis, meningitis, urinary tract infections and soft tissues.
Klebsiella pneumoniae produces a number of virulence factors that are involved in pathogenesis. These include: siderophores, O antigens, lipopolysaccharides, and capsules.
The irp-1 gene is the number one protein regulator of iron. This gene is responsible for controlling intracellular iron metabolism and can regulate the body's iron homeostasis by regulating histidine. The aim of this study was to evaluate the frequency of irp-1 and irp-2 genes in clinical isolates of Klebsiella pneumoniae.
Methods: In this study, 100 clinical isolates of Klebsiella were collected during 7 months. Samples taken in the laboratory were evaluated differentially and biochemically. Antibiotic susceptibility was assessed by disk diffusion method. DNA extraction was performed by boiling method and genes were identified by specific primers using PCR technique.
Results: Out of 100 samples, 63 samples (63%) were male and 37 samples (37%) were female. Of the total isolates, 83 isolates had resistance, of which the prevalence of irp-1 and irp-2 genes were 73.2% and 53.8%, respectively.
Conclusion: The results of this study show that the prevalence of irp-1 gene is higher than irp2 gene and it was found that there is an increase in resistance to genes involved in iron acquisition in the isolates.