Association of ART3 rs6836703 polymorphism with non-obstructive azoospermia in Iranian men.
Association of ART3 rs6836703 polymorphism with non-obstructive azoospermia in Iranian men.
Pouria Yousefi,1,*Amir amiriyekta,2Mehdi Shamsara,3Elmira Vanaki,4Morteza Daliri Joupari,5
1. National Institute of Genetic Engineering & Biotechnology 2. Royan Institute 3. National Institute of Genetic Engineering & Biotechnology 4. National Institute of Genetic Engineering & Biotechnology 5. National Institute of Genetic Engineering & Biotechnology
Introduction: According to the definition of the World Health Organization, if a couple does not have children within one year after unprotected sexual activity, they suffer from infertility, which affects infertility in both men and women. Various factors affect male infertility, about 15 to 30% of which are genetic abnormalities. It is also predicted that most unknown cases of infertility are based on a genetic defect. Studies on the ART3 gene have shown that it is associated with NOA. Therefore, in this study, we investigated the association of ART3 rs6836703 polymorphism with NOA in the Iranian infertile male population.
Methods: In this study, 100 genomic DNA samples of NOA individuals and 100 samples of control (fertile) individuals were received from Royan Research Institute, and then using Tetra ARMS-PCR technique, rs68367033 polymorphisms were examined in both groups.
Results: The results showed that the frequency of mutant A allele in the patient group was higher than the control group, but in both groups, the frequency of the normal G allele was higher than the mutant allele and no significant relationship was observed between the patient and healthy groups (P -0.067 and OR-0.672). Also, the findings about genotype showed that AA genotype is 16% in patients and 7% in control group and normal GG genotype is 45% in patients and 53% in control group, which indicates the higher frequency of mutated genotype in patients and The frequency of normal genotypes is higher in healthy individuals.
Conclusion: The study of rs6836703 polymorphic relationship in Iranian male population did not show a significant relationship with NOA.