Introduction: Background: Bladder cancer (BLCA) is a malignancy that is known to be fatal, with a 5-year survival rate of 77 percent. For the time being, the function of single nucleotide polymorphisms (SNPs) in associated noncoding RNAs (ncRNAs) with the genes implicated in BLCA is a new area of concern. The associations between one of the prognostic markers in BLCA and ncRNAs, as well as related SNPs, were investigated in the current study.
Methods: Method: First of all to display pertinence between BLCA and ncRNAs, a reliable prognostic marker was detect by searching from other articles. To investigate the highly differentially expressed genes and prognostic marker for BLCA, GEPIA2 was applied. LncRNASNP2 database was used to detect variants of these molecules related to the mentioned gene. miRNA assessment was proceeded by investigation of miRNAs by using miRWalk. Pathway analysis was performed based on other target genes of two mentioned miRNAs to assess whether they contribute to BLCA or not.
Results: Result: By investigation in different studies, P4HB was recognized as a prognostic marker that was upregulated in BLCA patients against normal. There is no validated evidence for lncRNA interaction with our candidate gene. Two miRNAs, hs-mir-4292 and hs-mir-4308 were detected that aimed P4HB as a target. pathway analysis of other target genes of chosen miRNAs, revealed that both of them participate in BLCA related pathways and malignancy of cancer.
Conclusion: Conclusion: Our finding indicated P4HB and its associated ncRNAs are able to participate in a prognosis diagnosis, and invasiveness of BLCA.
Keywords: in silico, BLCA, ncRNAs, miRNAs, prognostic marker