The frequecncy of Human Polyomavirus BK in colorectal cancer fecal samples
The frequecncy of Human Polyomavirus BK in colorectal cancer fecal samples
Mahbube Farbarin,1 Hoorieh Soleimanjahi,2,*Bita Bakhshi,3Zeynab Nasiri,4Kamal Fakhredini,5
1. Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 2. Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 3. Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 4. Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran. 5. Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Introduction: Polomaviruses are oncogenic in rodent models and readily
transform animal and human cells in-vitro. BKV is among this
family of viruses. BK virus genome encodes a transforming
protein known as Lareg T-Ag that intract with P53 and pRb
tumor suppressor proteins altering cell cycle control and
inducing malignant transformation. Thus there is a chance that
BK virus could potentially promoting some neoplasia such as:
colorectal cancer(CRC). CRC is the third most common cause of
cancer death in the world. The risk factors developing CRC is
associated with personal features such as age and gut
microbiota. Many human neoplasms are often initiated by
exposure to infectious agents.
Methods: Method: We assayed VP1 of BK virus in fecal samples, including 25
patients of CRC and 25 healthy cases. VP1 was detected by a PCR
technique.
Results: Result: Based on our research, positive results in PCR, were obtained
for 20% CRC group and just 4% sample in healthy group.
Conclusion: Conclusion: Taken together, PCR results for BK virus in patient
samples idicate that may ye a causal relationship between the
development of CRC and the virus existance. However the exact role of
the BK virus is still controversial and needs to further study.
Keywords: Keywords: BK virus, Colorectal cancer, VP1, PCR, Gute microbiota.