Introduction: Introduction: Cardiac progenitor cells (CPCs) are a heterogeneous group of cells distributed throughout the different parts of the heart. Under normal physiological conditions, these cells are to be quiescent and significantly do not contribute to renewal and rebuilding of cardiomyocytes. After heart failure, CPCs can be contributed in create new myocytes or vascular cells. Among these cells, C-kit+ cells have played a major role and greater potential other CSCs. In the other word, cellular aging plays an important role in cell function. The aim of the study was to evaluate cell survival of cardiac-derived C-kit+ cells.
Methods: Methods: About 3 male Rattus rats were euthanized using ketamine/Xylazine. Heart tissues were obtained and were then enzymatically dissociated using 0.075% collagenase type I. Next, the solution was neutralized by the FBS and cells were taken on Ficoll-Hypaque gradient centrifugation. Subsequently cells were incubated with C-Kit+ micro beads. Thereafter re-suspended cells were passed through one magnetic associated cell sorting (MACS) column. For purity assessment of C-Kit+ cells, flow cytometry was performed by florescence assisted cell sorting (FACS) Caliber. In the following, C-kit+ cells was cultured for 24, 48, 72, and 96 hours and the cells viability was investigated by MTT assay.
Results: Results: Flow-cytometric analysis showed that the 90% of isolated cells were expressed the C-Kit+ markers. The MTT results showed that the cell viability of cardiac C-kit+ cells decreased over time.
Conclusion: Conclusion: Taken together, it can be concluded that the survival rate of cardiac-derived C-kit+ enriched hematopoietic stem cells, like other stem/progenitor cells, changes over the time and requires special treatments to maintain growth rate. These findings could be effective in regenerative medicine.