Induction of apoptosis by galectin-9 in LNCaP cell line of prostate cancer
Induction of apoptosis by galectin-9 in LNCaP cell line of prostate cancer
Narges Zargar Balajam,1,*
1. Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences. Isfahan, Iran.
Introduction: Galectin-9, as a member of the lectin family with a beta-galactoside recognition domain, plays a significant role in inhibiting tumor cell proliferation. Prostate cancer is the second most common cancer in men and the sixth leading cause of death worldwide. In this study the effect of galectin-9 was investigated in LNCaP cell line of prostate cancer.
Methods: MTT viability test was applied to study the cell proliferation effect of galectin-9 in LNCaP cell line with various concentrations of galetin-9. Detection of apoptosis was conducted using the Annexin V-FITC/PI method.
Results: Our results showed that galectin-9 significantly reduced Cell viability in a dose-dependent manner ( P< 0.5). In addition, further analysis showed that this growth inhibition was related to apoptosis induction by galactine-9 in the LNCaP cell line.
Conclusion: In conclusion this study demonstrated that galectin-9 can inhibit cell proliferation and induce apoptosis in the LNCaP cell line. Thus, the inhibition of galectin-9 in tumor cells may be potentially a target in prostate cancer that needs to be explored in future.