The effect of curcumin on lung csf secretion and evaluation of its antioxidant enzyme activity
The effect of curcumin on lung csf secretion and evaluation of its antioxidant enzyme activity
saba zakeri,1,*Zahra Zamani ,2Sahel zakeri ,3Zahra Mehdipour ,4Karim Zamanfar ,5
1. Phd student of Clinical Biochemistry, kermanshah University of Medical Sciences, Kermanshah, Iran 2. 1. Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran. 3. Department of Biology, , Faculty of Basic Sciences, Islamic Azad University,Sanandaj,Iran 4. Department of Biology, , Faculty of Basic Sciences, Islamic Azad University, Karaj, Iran. 5. Department of Medical sciences, Clinical Research Development Unit of Imam Reza Hospital, affiliated to Kermanshah University of Medical Sciences, Kermanshah ,Iran
Introduction: Curcumin, a yellow pigment from Curcuma longa, is a major component of turmeric and is commonly used as a spice and food-coloring material. It exhibits anti-inflammatory, antitumor, and antioxidant properties., Colony-stimulating factors (CSFs) are secreted glycoproteins that bind to receptor proteins on the surfaces of hemopoietic stem cells, thereby activating intracellular signaling pathways that can cause the cells to proliferate and differentiate into a specific kind of blood cell. Numerous studies have shown the antioxidant role of colony stimulating factor .The aim of the present study was Investigation of the Effects of two types of curcumin monomer and polymer on lung csf secretion through bone marrow cell colonization and evaluation of its antioxidant activity in Balb mice in Balb / C mice.
Methods: In this study, we used Balb / C mice, which is one of the most suitable breeds for bone marrow culture. Bone marrow cells were obtained as a source of hematopoietic stem cells from the femur and tibia of mice. Mice lungs were also cultured as CSFs secreted tissue to prepare CSFs.
Results: Each experiment was repeated three times. Mean and standard deviation from the results were determined. SPSS test was performed by ANOVA method at the level of p <0.05 and the comparison between the results was significant. In the presence of curcumin monomer and curcumin polymer, the antioxidant enzymes, catalase, superoxide dismutase, glutathione peroxidase activity at certain concentrations increased , This increase is statistically significant for P <0.05 compared to the control and then fall down at more than this concentration.The results of this study are consistent with previous studies in which the effects of curcumin on antioxidant activity have been investigated.
Conclusion: in concentrations 30 µg/ml polymer curcumin and 25µg/ml monomer curcumin significantly secretion of CSF and the activity of the antioxidant enzymes catalase, superoxide dismutase, glutathione peroxidase increases And in higher concentrations have the opposite effect.