• Two layered nanofibrous mat with grape seed extract can promote wound healing in diabetic rat
  • fereshteh nejaddehbashi,1,* zeinab rafiee,2 mahmoud orazizadeh,3 vahid bayati,4 Ali asghar Hemmati,5 Mahmoud hashemitabar,6
    1. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    3. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute,Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    4. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute,Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    5. Medicinal Plant Research Center, School of pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
    6. Cellular and Molecular Research Center, Medical Basic Sciences Research Institute,Department of Anatomical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran


  • Introduction: Background:Despite special advances in regenerative medicine, wound healing is a challenging medical problem. The skin is the largest organ in the human body that has essential and important functions, so any damage to its normal structure should be treated as soon as possible.
  • Methods: Materials and methods: In this study PCL/SSD, PCL/Coll as two -layered nanofibrous mat were designed with electrospinning. In vitro tests including FE-SEM, Release test, MTT assay and EDX test were done and in vitro evaluation for their healing ability in a diabetic rat model of a full-thickness excisional wound was done. Post operation study including H&E and trichromasons were done on parrafine sections after 3, 7 and 10 days after transplantation The healing process was evaluated with macroscopic observation on 3,7 and 14 days' post wounding. Hematoxylin - Eosin and Trichromasons staining was done to evaluate tissue remodeling and collagen synthesis
  • Results: Results: This combination of nanofibrous mat with grape extract showed appropriate normal characteristics with desirable skin wound healing. Moreover, attachment and spreading of limbal stem cells on this nanofibrous composite was reported to be in an optimal condition. As well, in vivo evaluation demonstrated that full repaired skin had been observed on day 14 in the two-layered nanofibrous mat with grape extract group after being wounded.
  • Conclusion: Conclusion: The two-layered nanofibrous mat with grape extract could be considered as a suitable wound dressing of nanofibrous membranes in future in order to shorten healing time.
  • Keywords: Key words:grape seed extract,nanofibrous mat,electrospinning,diabetic rat, wound healing