Introduction: Breast cancer is the most important cause of cancer mortality among women that the study of its causative or aggravating factors seems necessary. In current study, the mRNA levels of PHB2 gene were evaluated in tumor and adjacent non-tumor tissues of 50 women diagnosed with invasive ductal carcinoma of breast.
Methods: RNX-Puls reagent was applied to isolate total RNA from tumor and adjacent non- tumor tissues of breast cancer patients. Thereafter, total RNA was converted to cDNA using Prime Script TM RT reagent kit. The mRNA levels of PHB2 were quantified by qRT-PCR and data was analyzed with paired sample t test. Furthermore, ROC curve analysis was performed to evaluate biomarker capacity of PHB2 in breast cancer tumor tissues.
Results: The significant low level of PHB2 mRNA was observed in tumor tissues of breast cancer patients compared with adjacent non-tumor tissues (P<0.05).
Conclusion: Low mRNA levels of PHB2 gene in breast cancer patients significantly discriminate the tumor from non-tumor tissues.