Effect of liposomes containing nettle essential oil on Pseudomonas aeruginosa and Escherichia coli as nosocomial infections
Effect of liposomes containing nettle essential oil on Pseudomonas aeruginosa and Escherichia coli as nosocomial infections
Mostafa rahimi nasab,1,*Ruholla rahimi nasab,2Mohammad Javad Forouzani-Moghaddam,3
1. Bachelor of Biotechnology, Yazd University, Yazd, Iran 2. Bachelor of Biotechnology, Yazd University, Yazd, Iran 3. Msc of Food Microbiology, School of Paramedical, Iran University of Medical Sciences, Tehran, Iran
Introduction: The aim of this study was to investigate the antibacterial effect of liposome containing nettle essential oil. For this purpose, the lipid system containing nettle essential oil has been synthesized for nosocomial infections Pseudomonas aeruginosa and Escherichia coli antibacterial methods.
Methods: The type of study is laboratory research. The nanoparticle synthesis method is Mozaffari method. Particle characterization has been performed in terms of size and charge with DLS and morphology with the Atomic Force Microscope (AFM) and the amount of loading and release with the spectrophotometer. MIC tests were then performed to evaluate the performance of nanoparticles containing clove essential oil on Pseudomonas aeruginosa and Escherichia coli.
Results: The average particle diameter was 46 nm and its zeta potential was -13.8 mV. The loading rate in nanoparticles was 76%, which was calculated by reading the absorption of light from the standard Trachyspermum Copticum curve. The minimum inhibitory concentration (MIC) of Pseudomonas aeruginosa and E.coli for nanoparticles was 62.5 and 31.25 mg / ml.
Conclusion: Nanoparticles containing nettle essential oil kill nosocomial infections Pseudomonas aeruginosa and Escherichia coli and can be used as antibacterial nano-systems.