• Identification of Antibacterial and Antifungal features of Tabrizicola aquatica gen nov., sp isolated from Qurugol Lake in mountain region of Azerbaijan.
  • tahereh ebrahimi,1 vahideh tarhriz,2,* azita dilmaghani,3
    1. Department of Nano-Biotechnology, Pasteur Institute of Iran, Tehran, Iran
    2. Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
    3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.


  • Introduction: Recently, resistant pathogenic microorganisms have become increasingly wide spread. The search for novel natural antibiotics is a viable solution to this problem. For this aim we investigated the antimicrobial ability of Tabrizicola aquatica, the novel bacterium isolated from Qurugol Lake located nearby Tabriz city, Iran.
  • Methods: The antimicrobial properties of Tabrizacola aquatica was investigated using well diffusion test. Tabtizicola aquatica was incubated at 40℃ in shaking incubator at 150 rpm for 14 days. The culture was centrifuged to obtain cell free supernatant, which was sterilized using 0.2 μm filter paper and lyophilized. Microorganisms were lawn and then wells were prepared over the agar plates. About 100 ml of the diluted lyophilized supernatant was added to the wells. The plates then were incubated at 37℃. After 48 hours, antimicrobial activity was defined by measuring the inhibition zone diameter.
  • Results: The bacterial filtrates had considerable antagonistic effect against Escherichia coli, Rhizobium radiobacter, Pseudomonas syringae, Erwinia amylovora, Botrytis cinerea, Neurospora crassa and Fusarium oxysporum. However, the filtrates did not show any inhibitory action on the Aspergillus flavus and Klebsiella pneumonia. The supernatant decreased the growth zone on Streptococcus aureus, Pseudomonas aeruginosa, Shigella flexneri, Xanthomonas camoestris and Bassilus cereos. The result of MIC against pathogens was found for Neurospora crassa in the 50 μg/mL.
  • Conclusion: The results, suggested that Tabrizicola aquatica and similar bacteria can be helpful to control freshwater natural water sources from pathogenic microorganism. Moreover, microbial natural products are still the most promising source of new antibiotics. Our results point out a scope for characterization of the metabolites and could be a candidate in the identification of novel antibiotics.
  • Keywords: Tabrizicola aquatica, Freshwater bacterium, Antibacterial and Antifungal Activity