Immunohistochemical expression of CB1 receptor in the liver of patients with HBV related-HCC
Immunohistochemical expression of CB1 receptor in the liver of patients with HBV related-HCC
Bita Moudi,1,*Zahra Heidari,2Hamidreza Mahmoudzadeh-Sagheb,3Mohammad- Reza Mohades,4
1. 1Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran 2. 1Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran 3. 1Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran 4. Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
Introduction: The most common cause of HCC is HBV infection, which has a high death rate in the world. This study aimed to examine the immunoexpression of CB1 receptor in the liver of patients with HBV related-HCC in comparison with HCC, chronic HBV and healthy people
Methods: Participants in this case- control study were patients with only chronic hepatitis B (HBV=40), only hepatocellular carcinoma (HCC=41), HBV related-HCC (HBV + HCC=40) and healthy control group (C=30). Tissue expression of CB1 at the protein levels were studied using immunohistochemical method.
Results: All groups were statistically significant in terms of expression of CB1 protein (p<0.001). The expression levels of CB1 in liver tissue of HBV and C groups were not statistically significant (p=0.072). The expression level of CB1 in liver tissue of HBV related-HCC and HCC groups had a statistically significant increase compared to the C and HBV groups (P<0.001). Also, the CB1 expressions in liver tissues of HBV related-HCC and HCC groups were statistically significant (P = 0.008). Sensitivity and specificity of immunohistochemistry test in the diagnosis of HCC using CB1 were 63.4 and 91.2, respectively. Also, positive and negative predictive values were 90.0 and 65.1 %, respectively. There was no relationship between the expression of CB1and other clinicopathological variables (p<0.05).
Conclusion: The present findings reveal a tumor promoting function of the CB1 receptor in HCC. CB1 is also a pathological valuable factor in identifying the pathway of inflammation during infection.