Cannabinoid receptor type-1 and its correlation with CB1 gene polymorphism-1359G/A in ectopic pregnancy compared to the control group
Cannabinoid receptor type-1 and its correlation with CB1 gene polymorphism-1359G/A in ectopic pregnancy compared to the control group
Bita Moudi,1,*Zahra Heidari ,2Azam Asemi-Rad,3Hamidreza Mahmoudzadeh-Sagheb,4Nadia Sheibak,5Marzie Ghasemi,6
1. 1Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran 2. 1Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran 3. Department of Anatomical Sciences, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran 4. 1Infectious Diseases and Tropical Medicine Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran 5. Department of Histology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran 6. Moloud Infertility Center, Ali ibn Abitaleb Hospital, Zahedan University of Medical Sciences, Zahedan, Iran
Introduction: Ectopic Pregnancy (EP) is one of the most important causes of maternal mortality. This study aimed to evaluate the immunohistochemical expression of the cannabinoid receptor type 1 (CB1) and its association with CB1 -1359G/A gene polymorphism (rs1049353) in the fallopian tubes in ectopic pregnancy compared to controls.
Methods: In this case-control study, 100 women with ectopic pregnancy (cases) and 100 women that underwent abdominal surgery due to the hysterectomy or uterine tubal ligation (healthy controls) were included. Genotyping of CB1-1359G/A polymorphism, tissue expression of CB1 at the protein and mRNA levels were studied using restriction fragment length polymorphism, immunohistochemical (IHC) method, and quantitative real-time polymerase chain reaction (qRT-PCR) analysis.
Results: Genotyping showed that in EP, the frequency of AA, AA+AG genotypes, and A allele was significantly higher than healthy control subjects (P=0.001).
Also, patients with EP had significantly increased IHC expression of CB-1 compared to the control samples (P = 0.016). Patients with AA and AG genotypes had a significantly higher IHC expression of CB-1 compared to the GG genotype. Quantitative real-time PCR analysis showed that patients with EP had significantly increased expression of CB-1 compared to the control samples (P<0.001). Patients with AA and AG genotypes had higher significant mRNA expression of CB-1 compared to the GG genotype.
Conclusion: CB1 is likely to be effective in creating innate immunity in humans and can affect the process of EP in the fallopian tube. CB1 is also a pathological valuable factor in identifying the pathway of inflammation during ectopic implantation.