• Frequency of polymorphism (rs28665122)-105 G>A in selenoprotein S gene among patients with type 2 diabetes and its correlation with selenoprotein S and glucose levels and oxidative stress status
  • Akram Mehrzad Selakjani,1,* Mohsen Firoozray,2 Mohammad Hossein Hasani,3 Sahar Moghbelinejad,4 Alireza Farrahi,5 Masoud Tahani,6
    1. Student, Clinical biochemistry, Medical faculty, Shahrood Azad university, Email: akram919mehrzad@yahoo.com, ORCID iD: 0000-0001-7938-6759
    2. professor, Clinical biochemistry Department, Medical faculty, Shahrood Azad university.
    3. Department of Interal medicine, Islamic Azad university, Shahrood Branch, Shahrood, Iran
    4. Cellular and Molecular Research Center, Qazvin university of medical sciences, Qazvin, Iran.
    5. Student, Clinical biochemistry, Medical faculty, Shahrood Azad university, ORCID iD: 0000-0002-3112-8918.
    6. Student, Clinical biochemistry, Medical faculty, Shahrood Azad university.


  • Introduction: Type 2 diabetes (T2D) is a type of metabolic disorder characterized by high blood glucose levels in conditions of insulin resistance and relative insulin deficiency. Oxidative stress can play a role in the development and progression of this disease. Selenoprotein S is an essential antioxidant protein. Accordingly, the purpose of this study was to evaluate the role of selenoprotein S gene (rs28665122)-105 G>A polymorphism in T2D.
  • Methods: The present case-control study was conducted on 115 T2D patients and 109 healthy individuals from the Diabetes clinics in Tehran (Iran). After collecting venous blood samples, the measured parameters included glucose level, malondialdehyde (MDA) content, total antioxidant capacity (TAC), and HbA1c level. Serum selenoprotein S (SEPS1) level and lipid profile were measured as well. The SEPS1 gene promoter polymorphism (rs28665122)-105 G>A was analyzed by RFLP technique and the correlation between genotype and serum parameters was also evaluated.
  • Results: The mean total cholesterol concentration was not significantly different between the patient and healthy groups; however, the other studied biochemical parameters showed a significant difference between the two groups. It was also revealed that the subjects with GA and AA genotypes at rs28665122-105 G>A with OR = 0.985 and OR = 4.044 had a higher risk of diabetes than the normal genotype, respectively. Among the serum parameters studied, only blood glucose concentrations, HbA1c and HDL-C had significant correlations with different genotypes.
  • Conclusion: The results of this study indicate the correlation between rs28665122 polymorphism and T2D and also the effect of allele A of this polymorphism on T2D incidence.
  • Keywords: polymorphism, rs28665122, selenoprotein S, type 2 diabetes, oxidative stress