Studying the impact of estradiol on CD90 positive spermatogonial stem cells viability

Studying the impact of estradiol on CD90 positive spermatogonial stem cells viability
Fatemeh Sokuti Nasimi ,¹ Saber Zahri,² Behnam Hashemi,³ Shahin Ahmadian,⁴ Reza Rahbarghazi ,⁵ Mahdi Mahdipour,^6,*
2. Department of Biology, Faculty of Science, University of Mohaghegh Ardabili, Ardabil, Iran
3. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran
4. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, 5165665811, Iran
5. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
6. Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Introduction: Until now, there are controversies about effect of estradiol on regulation of reproductive function and fertility in males. In this study we investigated the possible adverse/positive effects of estradiol on male murine germ cells in-vitro after seven days.
Methods: Following testicle tissue sampling, single- cell suspensions were harvested using 0.25% Trypsin/EDTA solution. CD90 positive cells were enriched using magnetic activated cell sorting technique. CD90 positive cells then were treated with different concentrations of estradiol ranged between 500μg/ml and 0.01ng/ml. After 7 days of treatment, cell survival rate was monitored using conventional MTT assay.
Results: Our findings revealed that, estradiol could potentially increase the viability of CD90 positive cells in a dose dependent meaner compared to the non-treated control cells (P<0.05).
Conclusion: These results showed that, estradiol could have positive effect on survival of CD90 positive cells in-vitro in male germ cells.
Keywords: CD90 positive cells; spermatogonial stem cells; Estradiol; Cell vaibility