The Effect of Sinapic Acid Treatment on Acute Anxiety Behavior in Adult Male NMRI Mice

The Effect of Sinapic Acid Treatment on Acute Anxiety Behavior in Adult Male NMRI Mice
shahrzad rabanifard,^1,* Shahrbanoo Oryan,² Akram Eidi,³
1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2. Department of Biology, Faculty of Science, Kharazmy University, Tehran, I.R. Iran
3. Akram EidiDepartment of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Introduction: Sinapic acid, a phenolic acid is a cinnamic acid derivative. The elevated plus-maze (EPM) and open-field test have broadly been used to investigate anxiolytic and anxiogenic compounds. The present study investigated the effect of sinapic acid treatment on acute anxiety behavior in adult male NMRI micein the EPM and open-field apparatus
Methods: . Animals were divided into five groups: control group and sinapic acid groups (5, 10 and 20 mg/kg, i.p.) and diazepam (0.75 mg/kg). During the test period, the number of open- and closed-arm entries, and the time spent in open of the EPM were recorded. Also, the anxiety behavior of animals was evaluated by open-field apparatus. Additionally, the GABA A/benzodiazepine (BDZ) mediation of the effects of sinapic acid was evaluated.
Results: Our results showed that administration of sinapic acid and diazepam significantly increased number of entries and time spent in open-arm of the EPM, whereas sinapic acid and diazepam significantly decreased number of entries and time spent in closed-arm of the EPM. The effects of sinapic acid in the elevated plus-maze were not altered by picrotoxin or flumazenil pretreatment.
Conclusion: The results of the present experiment indicate that sinapic acid may have an anxiolytic profile in mice behavior in the EPM and open-field test, which were not mediated by GABA A/BDZ neurotransmission.
Keywords: Sinapic acid, Anxiety, Mice, EPM