Antimicrobial effect of new compounds sulfadiazine-derived drug on Pseudomonas aeruginosa

Antimicrobial effect of new compounds sulfadiazine-derived drug on Pseudomonas aeruginosa
Parisa khiabanirad,¹ Sorour Aalipanah,^2,* Shohreh Nasiri poroj,³ Neda Zamani,⁴ Mahdieh ramezani,⁵ Koroush Motaghinia,⁶
1. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
2. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
3. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
4. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
5. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
6. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
Introduction: Pseudomonas aeruginosa is one of the most important causes of nosocomial infections that can cause widespread infections in patients with defective immune systems. Burn wounds are a good place for resistant infections and Pseudomonas aeruginosa is the most common bacteria that cause these infections. Therefore, research on obtaining effective drugs seems necessary. Sulfadiazine is a broad-spectrum sulfonamide antibiotic that inhibits bacterial growth and replication by inhibiting folic acid synthesis. This drug is used in combination with silver as a burn ointment. There are several studies on the use of this drug in combination with other drugs. The aim of this study was to evaluate the antibacterial effects of a new drug derived from sulfadiazine (sulfadiazine + 4-diamino benzaldehyde) on Pseudomonas aeruginosa.
Methods: For this purpose, 0.2 mmol of sulfadiazine with 0.2 mmol of 4-dimethylaminobenzaldehyde with 10 ml of acetic acid solvent at 110 ° C was refluxed on the heater for 10 hours at 200 rpm. The resulting orange precipitate was crystallized with 2-propanol to obtain a pure precipitate. Finally, the structure of the synthesized compound was confirmed by determining the melting point, C-NMR, H-NMR, TLC and IR. Then the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of the synthesized drug on Pseudomonas aeruginosa were determined exerting microdilution method on Müller-Hinton broth medium.
Results: Different color, melting point and the results of C-NMR, H-NMR, TLC and IR indicate the new structure of the synthesized drug. The MIC and MBC of sulfadiazine-derived drug on Pseudomonas aeruginosa were 1 and 2%.
Conclusion: The synthesized drug effectively has antibacterial activity which can be used in pharmacy and treatment of infections caused by this bacterium.
Keywords: Sulfadiazine, Reflux method, Pseudomonas aeruginosa, Drug resistance, Antimicrobial effect