Wet Disc Testing of complex Mafenide acetate and cobalt Against Bacteria

Wet Disc Testing of complex Mafenide acetate and cobalt Against Bacteria
Mahdieh Ramezani,¹ Shohreh Nasiri poroj,^2,* Sorour Aalipanah,³ Elham Taghinia,⁴ Parisa Khiabanirad,⁵ Koroush Motaghinia,⁶
1. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
2. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
3. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
4. Department of Microbiology, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
5. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
6. Department of medicinal chemistry, Faculty of Life Sciences, Islamic Azad University, North Tehran Branch
Introduction: Today, due to increased infections, antimicrobial agents and coatings are necessary. Increasing bacterial resistance is attributed to drug abuse so that more potent antibiotics are needed. They also require topical disinfectant to reduce the occurrence of drug resistance. In addition to the development of antimicrobial agents, it is necessary to effectively inhibit the growth of microorganisms. Some materials such as Mafenide acetate and cobalt have many applications in industry and health because of having antibacterial properties. This study aimed to investigate the antimicrobial properties of Mafenide acetate and cobalt complex that have this property.
Methods: For this study, 0.1 mmol of mafenide acetate and 0.1 mmol of cobalt nitrate in 10 ml of ethanol solvent were refluxed for 3 hours. The reaction progress was monitored using thin-layer chromatography (TLC) in normal hexane: ethyl acetate tank in a ratio of (1: 4). The solvent was evaporated, and the precipitate was crystallized with 2-propanol solvent. In this study, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of nanoparticles of zinc oxide were determined using well assay method.
Results: For each antimicrobial agent, the MIC and MIC were determined from the broth microdilution results and the mean zones of inhibition were calculated from the agar well diffusion. According to the results of this study, the MIC values of this complex for Staphylococcus aureus were 3% and the MBC values for Staphylococcus aureus were 6% concentration.
Conclusion: The present study showed that this complex can be used as a deterrent against the pathogens of the materials and avoid contamination. Finally, the results obtained in this study suggest that the use of this complex as an antibacterial agent in industry and health can successfully inhibit some of the most dangerous and frequent pathogens.
Keywords: Antibacterial, staphylococcus aureus, Microdilution,