Efficacy of Using probiotic lactobacilli with Antagonistic Activity against Pathogens of Wound Infections

Efficacy of Using probiotic lactobacilli with Antagonistic Activity against Pathogens of Wound Infections
Mohammad Abootaleb,^1,* Narjes Mohammadi Bandari,² Nazila Arbab Soleimani ,³
1. Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
2. Department of Biology, Qom Branch, Islamic Azad University, Qom, Iran
3. Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran
Introduction: Burns are the most common type of trauma with a high mortality rate worldwide. Using modern and natural medicines, especially probiotic products have recently been regarded for cutaneous wounds healing. The present study was designed to investigate the effects of Lactobacillus plantarum and Lactobacillus casei on wound healing and antimicrobial activity of two probiotic lactobacilli against P. aeruginosa.
Methods: In this study, Anti-adhesion activity considered by the glass slide method and the Anti-adhesion substances in cell-free supernatant (CFS) were quantified by the HPLC method.. After induction of the second-degree wounds, MDR P. aeruginosa injected subcutaneously directly on the burn. The animals were divided into 5 groups. The supernatants were sprayed for treatment every day and the evaluation of wound healing was conducted
Results: Based on our findings, lactobacilli bacteria exerted good anti-adhesion effects on P. aeruginosa which HPLC analysis indicated that their inhibitory effect can be due to their main organic acids. The effect of treatments on fibroblastic cells showed that the treated group by supernatants of L. plantarum and Lactobacillus casei had the most number of fibroblastic cells compared with the non-treated group. Moreover, the bacteria increased the rate of fibroblastic cells, re-epithelialization in the wound area and the largest thickness of the epidermis and dermis layers. Antimicrobial activity of lactobacilli bacteria against MDR P. aeruginosa was determined by prevents infection.
Conclusion: These findings showed that probiotic bacteria specially significantly reduced inflammation and they can treat a P. aeruginosa infection in a second-degree burn.
Keywords: Wound healing, Cutaneous wound, Probiotics, Burns, P. aeruginosa