3rd International Congress on Biomedicine (ICB2019)
Antimicrobial activity of different concentrations of leech saliva on bacterial growth by disk diffusion and micro MIC
Antimicrobial activity of different concentrations of leech saliva on bacterial growth by disk diffusion and micro MIC
zeynab jafari bidhendi,1,*mostafa jafarpour,2
1. MS.c., Department of Microbiology, Tonekabon Branch, 1Islamic Azad UniversityTonekabon, Iran. 2. Assistant Professor, Department of Microbiology, Tonekabon Branch, 1Islamic Azad UniversityTonekabon, Iran.
Introduction: The challenge of antibiotic resistance today due to its widespread use against infectious diseases is a significant issue. Accordingly, the production of natural antimicrobial agents from animal sources is interesting , Recently, some researchers have reported on the antibacterial effects of saliva and leech body, The aim of this study was to evaluate the antimicrobial activity of Hirudo orientalis native Caspian leech saliva extract against Gram-negative and Gram-positive pathogenic bacteria.
Methods: The leeches studied were collected from the running waters of Tonekabon agricultural fields and were kept at room temperature after specifying Orientalis species in plastic containers containing non chlorinated water, For a period of twelve weeks, starvation was fed by a phagostimulatory solution (0.100 M Arginine in 0.15 M NaCl) and the saliva was filtered off with 45% microl paper
, and at 4 ° C for 10 min With 3000 centrifugation rounds, the supernatant is a raw extract of leech saliva that has been evaluated by disk diffusion and micro-microassays for antimicrobial activity against pathogenic bacteria.
Results: The most antagonistic effect of the extract was by disk diffusion method on Escherichia coli with a growth inhibition zone of 10.2 +_ 2 mm , Two other gram-negative bacteria studied, Pseudomonas aeruginosa and Klebsiella pneumonia , showed no measurable inhibition zone and no growth halo was detected against MRSA. In Micro Mic method with the numbers of 1.6, 25 , 25.6 antimicrobial activity against Escherichia coli bacteria with PTCC1330, Klebsiella pneumoniae with PTCC 9310, Pseudomonas aeruginosa with PTCC 1811 and PTCC 1764 was observed, respectively, and no activity against MRSA PTCC 1764 Not found to match the disk release method.
Conclusion: The wide biodiversity of leech species in different parts of the world motivates the evaluation of the antibacterial activity of the native Cashew leech saliva extract. Analysis of the results shows appropriate antibacterial activity against pathogenic Gram-negative bacteria especially Escherichia coli , If there was no antagonistic activity against the gram-positive bacteria studied by MRSA, this may be due to the presence of different antimicrobial compounds in different leech species in different parts of the world. Due to the presence of antibacterial agents in the salivary extract of native Caspian leeches, extraction and purification of thes compounds can provide a suitable alternative to fabricated antibiotics.