• Investigation of the apoptotic impact of Human amniotic membrane-derived stromal cells (hAMSC) on breast cancer cells
  • yasaman aliasgharisakha,1 Amin EbrahimiSadrabadi,2 Arsalan Jalili,3,*
    1. developmental of biology
    2. Cancer Biomedical Research Center, Tehran, Iran (CBC)
    3. Hematopoietic Stem Cell Research Center, Shahid Beheshti University of Medical Sciences


  • Introduction: It is about so many years which breast cancer are in the top 3 highest mortality rate among women's tumors. There were an estimated 1.7 million cases of breast cancer and 521,900 deaths in 2014, accounting for ~20% of all cancer diagnoses and 15% of all cancer-associated deaths in women. Several studies have shown dysregulation of the molecular machinery of apoptosis, the major programmed cell death pathway in breast malignancies. Thus, there is an unmet need to search for alternative cell therapy, which induces apoptosis. The immunomodulatory properties of MSC have been approved. Accumulating evidence suggests that mesenchymal stem cells are recruited to the tumor microenvironment. Several studies suggest that MSCs secrete that perform as mediators in the tumor niche and play several roles in tumorigenesis, angiogenesis, and metastasis. In contrast, other studies are supporting the tumor-suppressing effects of MSC. The objective of this study is to examine the apoptotic effect of AMMSC on breast cancer.
  • Methods: The MSCs have been extracted from the amniotic membrane. The breast cancer cell lines (MCF-7) have been co-cultured with MSCs. The MTT assay was used to determine the proper inhibitory concentration of MSC on tumor cells. The cell cycle and apoptosis have been investigated by expression of p53, caspase 1, 3, and 4 genes by real-time PCR, respectively.
  • Results: According to the MTT result, after 48 hours, cell death has been increased. The expression level of p53, caspase 1, 3, and 4 was higher than control cells.
  • Conclusion: This study has proved that MSCs isolated from the amniotic membrane play a role in reducing the proliferation and inducing apoptosis of breast cancer cells. The upregulation of P53, Caspase 1, 3, and 4, which increase the apoptotic phenomenon manifest the impact of hAMSC on breast cancer cells.
  • Keywords: hAMSC, Breast Cancer, Apoptosis