3rd International Congress on Biomedicine (ICB2019)
The effect of fibrin and fibrin/ polyurethane/ multiwall carbon nanotubes hydrogels, as scaffolds for neural tissue engineering, on human endometrial stem cells viability
The effect of fibrin and fibrin/ polyurethane/ multiwall carbon nanotubes hydrogels, as scaffolds for neural tissue engineering, on human endometrial stem cells viability
Elham Hasanzadeh,1Somayeh Ebrahimi-Barough,2Narges Mahmoodi,3Houra Nekounam,4Fatemeh Asghari,5Jafar Ai,6,*
1. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Mazandaran University of Medical Sciences, Sari, Iran 2. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran 3. Sina Trauma and Surgery Research Center, Tehran University of Medical Sciences, Tehran, Iran 4. Department of Medical Nanotechnology, School of Advanced Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran 5. Department of Medical Nanotechnology, School of Advanced Technology in Medicine, Tehran University of Medical Sciences, Tehran, Iran 6. Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
Introduction: In the central nervous system (CNS) disorders, unlike the peripheral nervous system (PNS), neurons have less ability to regenerate the neural tissues. Recently, tissue engineering has provided a new medical approach as an alternative to tissue transplantation or peripheral nerve grafting. Natural hydrogels such as fibrin gel can provide an environment that promotes cell proliferation, attachments, and migration. One limitation of hydrogels is poor mechanical properties as a scaffold. For this reason, incorporation of hydrogels with nanofibers, for the fabrication of nanofibrous hydrogels, can improve their mechanical properties to create composites that mimic the natural extracellular matrix (ECM). The purpose of the study is to evaluate the effects of fibrin and synthesized nanofibrous hydrogel scaffolds on human endometrial stem cell viability.
Methods: Human endometrial stem cells (hEnSCs) were isolated from endometrial biopsies and characterized in passage 3 using flow cytometry. These cells were cultured in Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12 (DMEM/F12) containing 10% fetal bovine serum (FBS) and passage 3 cells were used in experiments. To synthesis fibrin/ polyurethane/ multiwall carbon nanotubes composite the electrospun polyurethane fibers were cut into small pieces and mixed with fibrin gel. Finally, hEnSCs were cultured in fibrin and fibrin/ polyurethane/ multiwall carbon nanotubes hydrogel scaffolds, and then 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis and 4′,6-diamidino-2-phenylindole (DAPI) staining were used to determine cell viability.
Results: The results of MTT assay indicate no sign of cytotoxicity and showed a higher proliferation level and viability of hEnSCs in the fibrin/ polyurethane/ multiwall carbon nanotubes hydrogels compared to the fibrin hydrogels. Furthermore, according to the results of DAPI staining, a large number of hEnSCs were entrapped into the fibrin and fibrin/ polyurethane/ multiwall carbon nanotubes hydrogels that confirm our MTT results.
Conclusion: The findings of this study demonstrate that our fabricated fibrin/ polyurethane/ multiwall carbon nanotubes hydrogel can be employed as a suitable biomaterial scaffold for neural tissue engineering that supports cell proliferation and provided an appropriate microenvironment to enhance cell viability.