• Loading efficiency of miR-101 plasmid on Spion/PEI nanoparticles
  • Delaram sadat razavi,1 S.A hassan shahzadefazeli,2,*
    1. .MSc Student of Cellular and Molecular Biology, Biology Department, Sciences Faculty, Science and Arts University, Yazd, Iran.
    2. Department of Genetics, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran


  • Introduction: Gene Therapy is a set of therapies that treat the disease by repairing and fixing the gene. In the past, all kinds of gene therapy have relied on a vector for transferring the therapeutic agent into the cell, but other methods have now been proposed. Several viral and non-viral safe vectors have been devised and used to successfully treat some inherited diseases, immune defects, ocular and cancer. Viral vectors are suitable for gene therapy for diseases that require long-term gene expression. In this method, nanoparticles are first made of magnetic active substance (such as iron oxide) and after modification of their surface structure by effective functional groups and effective absorption of the drug onto it, they are directed to the target tissue by applying an external electromagnetic field. This will put the drug or gene in question into the tissue exactly. Iron oxide nanoparticles have been shown to be highly potent in the exchange of material between tissue and blood after ischemia and cellular protection. It also increases cell proliferation on proteins and nucleic acids. Polyethylene imine is a cationic polymer used as a non-viral carrier in gene therapy. By coating the iron oxide nanoparticles with polymer, the researchers succeeded in developing a new, stable, low-toxicity drug system that could be effective in treating cancer. These nanoparticles have shown positive results on laboratory mice. In particular, the application of these nanoparticles has focused on DNA that acts as a carrier for genes and drugs.
  • Methods: We loaded different concentrations of the gene on different concentrations of nanoparticles. Spion*PEI/Mir plasmid=w/w Different ratios of Spion-PEI / plasmid (1, 0.5, 0.25, 0.125. 0.06) on 2% agarose gels were investigated to study the interaction of nanoparticles and plasmid DNA.
  • Results: In this study, we obtained a nanoparticle-to-gene weight-to-weight ratio of nanoparticles by measuring the concentration of nanoparticles as well as determining the concentration of plasmids containing miR by a nanodrop machine
  • Conclusion: The results show that the amount of gene required on the appropriate amount of nanoparticles is that according to observations and experiments, a ratio of 150 ng nano particles to 300 ng of plasmid gene is required for complete loading of the gene and in this amount the amount of free gene is not observed in gel electrophoresis.
  • Keywords: nano particles,spion,gene therapy