• The evaluation of the antibiofilm activity of Niosome Nanoparticles with cefazolin antibiotic against Biofilm of Methicilin Resistant Staphylococcus aureus (MRSA) isolates
  • mahsa adibi,1,* morvarid shafiei,2 parisa shirvani,3 alireza kheirollahi,4 mohammad shams,5 mohsen chiani,6
    1. Department of Microbiology, Faculty of Science, Alborz University, ghazvin, Iran and Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
    2. Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
    3. Department of Microbiology, Faculty of Science, Alborz University, ghazvin, Iran and Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
    4. Department of medicine, faculty of medical sciences, university of tabriz,tabriz, Iran
    5. Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
    6. Department of Nanobiotechnology, Pasteur Institute of Iran, Tehran, Iran


  • Introduction: Methicillin resistant S.aureus (MRSA) has the propensity to form biofilms and causes significant mortality and morbidity in the patients with wounds. Nanoparticles are considered as a promising way to overcome the infections therapeutic problems caused by S.aureus. The aim of this study was to evaluate the antibiofilm activity of Niosome nanoparticles with cefazolin antibiotic against biofilm of S.aureus isolates.
  • Methods: The twenty five clinical isolates of MRSA were collected from patients with pressure and diabetic ulcers who were referred to Loghman Hospital of Tehran- Iran. The biochemical (coagulase, Mannitol salt agar, catalase, cefoxitin disk diffusion test) and Molecular (PCR) tests were used to detect MRSA. Cefazolin_eluting noisome nanoparticles were prepared using the thin film hydration method and were characterized by X- ray diffraction, Zeta potential measurement and TEM. The effect of the Cefazolin_eluting noisome nanoparticles was evaluated against one, three and five day old biofilms using TTC and Crystal violet methods.
  • Results: The round shaped cefazolin eluting_niosome nanoparticles, as observed in the TEM images, had diameter of 50-100 nm. The cefazolin eluting_niosome nanoparticles have eliminated one, three and five day old biofilms of MRSA at the concentrations of 62.5 µg/mL, 125 µg/mL and 250 µg/mL, respectively
  • Conclusion: Antibiotic eluting- noisome can be promising for the treatment of MRSA biofilm related infections
  • Keywords: cefazolin eluting_niosome nanoparticles, methicillin resistant S.aureus (MRSA), Biofilm