3rd International Congress on Biomedicine (ICB2019)
A novel soft tissue fabricated using carboxymethyl chitosan/diopside-Zn-doped α -Fe2O3 nanoparticles for promoting effects on wound healing
A novel soft tissue fabricated using carboxymethyl chitosan/diopside-Zn-doped α -Fe2O3 nanoparticles for promoting effects on wound healing
Atiyeh Raisi,1,*Maryam Shahali,2Azadeh Asefnejad,3Amirsalar Khandan,4
1. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran 2. Department of Quality Control, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran 3. Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran 4. New Technology Research Center, Amirkabir University of Technology, Tehran, Iran
Introduction: The skin is the most extensive organ in the body, that is subject to many injuries. Many people suffer from skin damage each year. Nowadays, it is less dependent on one's age, therefore, a significant number of young people suffer from disease. Therefore, many people need appropriate and effective treatment for wound healing and tissue damage. However, many improvements in this context, millions of people suffer from severe skin injuries each year. Therefore, trying to fix this problem and provide an appropriate, unlimited replacement is very important and valuable.
Methods: The present study aimed to prepare a nanocomposite dressing (NCD) composed of carboxymethyl chitosan, diopside, and Zn-doped α -Fe2O3 nanoparticles, which may promote wound healing and prevent from infection. Mentioned nanocomposite was prepared by freeze-drying (FD) technique.
Results: The NCD was subject to detailed analysis by scanning electron microscopy (SEM) analysis. The results showed that the wound dress was porous architecture and had porous interconnections. In addition, the cytotoxicity of this nanocomposite was determined by MTT assays for 24, 48 and 72 hours and showed no cytotoxicity toward growth of L929 fibroblasts and had good in vitro biocompatibility. Also, the bioactivity and biodegradability of the wounds were immersed in the simulated body solution (SBF) and phosphate buffer solution (PBS), respectively. Then, the mechanical properties (tensile strength) were evaluated using SANTAM machine according to the standard. The results revealed that NCD had good biodegradability and mechanical properties.
Conclusion: These results suggested that NCD composed of carboxymethyl chitosan, diopside, and Zn-doped α -Fe2O3 nanoparticle could enhance wound healing while having a good biodegradability.