3rd International Congress on Biomedicine (ICB2019)
Antibacterial and antifungal evaluation of Thymus vulgaris essential oil nanoemulsion
Antibacterial and antifungal evaluation of Thymus vulgaris essential oil nanoemulsion
Tina Khalilzade,1,*Maryam Hasan,2
1. Zanjan Pharmaceutical Biotechnology Research Center, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran 2. Department of Food and Drug Control, Faculty of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran
Introduction: In the recent years broad spectrum of essential oils properties have captured attention worldwide. This is due to their large variety of applications in different domains particularly as an antimicrobial agents. Nevertheless some of the current drawbacks including volatility poor water solubility as well as sensitivity to oxygen light and heat have turned their implementation to a challenging task. Hence introduction of nanoencapsulation technology appears to be a feasible approach as it enhances the physicochemical attributes of essential oils namely bioavailability solubilization and thermal stability. Here we show the antibacterial effects of nanoemulsion loaded with Thymus vulgaris essential oil employing a standard method mentioned in previous studies.
Methods: In the present study with taking into consideration authenticated antibacterial and antifungal properties of Thymus vulgaris essential oil efforts have been made to encapsulate the volatile substance in nanoparticles using high pressure homogenizing method adding tween 80 and span 60 as surfactants with the aim of attainment of desirable particle size and size distribution which was gauged by nano zetasizer. Morphological characterization of these particles was determined by Atomic force microscopy (AFM). Ultimately evaluation of prepared nanoemulsions antimicrobial effectiveness against standard bacteria and fungi e.g. Staphylococcus aureus Salmonella typhi Enterococcus faecalis Micrococcus luteus Bacillus subtilis Candida albicans was performed by measuring diameter of inhibition zones.
Results: Assessment of morphological features established spherical shape observed by Atomic force microscopy (AFM) and analytical outcomes obtained by Nano Zetasizer revealed regular distribution of nanoparticles with average size of 175 nm and PDI of 0/317. Further in vitro drug release demonstrated an initial burst release followed by constant release of essential oil. Comprehension of zone inhibition diameters related to the essential oil effect with presents antibiotics demonstrated satisfactory findings. The highest sensitivity belongs to Candida albicans which showed 39/5 ± 1 millimeter zone inhibition whilst that of Salmonella typhi was merely 15 ± 1 millimeter.
Conclusion: The result of this experiment illustrates that nanoemulsion loaded thymus vulgaris essential oil can be advantageous as it can be a solution for mentioned latent problems of Thymus vulgaris essential oil. Further the prepared nanoemulsion showed significant antimicrobial effect and have great potential in drug delivery.