3rd International Congress on Biomedicine (ICB2019)
Effect of zinc oxide nanoparticles synthesized by the green method from the extract of the hyssopus officinalis plant on skin tissue in order to determine the cytotoxicity in vitro
Effect of zinc oxide nanoparticles synthesized by the green method from the extract of the hyssopus officinalis plant on skin tissue in order to determine the cytotoxicity in vitro
Ghasem Rahimi Kalateh Shah Mohammad,1vahid pouresmail,2,*
1. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran 2. Assistant Professor, Department of Biochemistry., Faculty of Medicine, Islamic Azad University-Mashhad Branch (IAUM), IRAN
Introduction: Concerning the formation of zinc oxide nanoparticles that are synthesized both in chemical and green form, it has to be said that in the process of various plant extracts, which are very common today, it is completely safe and safe compared to chemical synthesis. Due to the use of zinc oxide nanoparticles (ZnO) in a wide range of products, including cosmetics, food packaging, imaging, etc., this increases the possibility of exposure to these nanoparticles by dermal, inhalable and Mouth gets In fact, the small size of nanoparticles penetrates the skin, absorbs the cells through the lymph channels and transsitosis into epithelial and endothelial cells into the bloodstream and lymph circulation to potentially sensitive target sites such as bone marrow, lymph nodes, spleen And the heart reaches. Also, access to the central nervous system and ganglion has been observed through transmission along with axons and nerve dendritic lesions. The biogenic synthesis of ZnO NPs by apply different plant extracts is also current nowadays. This green synthesis is fully safe and ecofriendly compared to chemical synthesis. Nanoparticles produced by plants are more stable and the rate of synthesis is faster than in the case of microorganisms. Moreover, the nanoparticles are more various in shape and size in comparison with those produced by other organisms. The advantages of using plant and plant-derived materials for biosynthesis of metal nanoparticles have interested researchers to investigate mechanisms of metal ions uptake and bioreduction by plants, and to understand the possible mechanism of metal nanoparticle formation in plants. NIH 3T3 mouse embryonic fibroblast cells were initiated from a cell line isolated in 1962 at the New York University School of Medicine Department of Pathology. 3T3 refers to the cell transfer and inoculation protocol for the line, and means “3-day transfer, inoculum 3 x 105 cells.” Using this protocol, the immortal cell line begins to thrive and stabilize in cell culture after about 20-30 generations of in vitro growth. George Todaro and Howard Green, the scientists who first cultured this cell line, obtained the cells from desegregated NIH Swiss mouse embryo fibroblasts. The cell line has since become a standard fibroblast cell line. This article elaborates the synthesis, properties, and applications of zinc oxide nanoparticles. During our work, we have synthesized ZnO NP via green routes using Hyssopus officinalis extract, giving a special emphasize on growth of NP at different temperatures. Optical properties of the synthesized NPs were measured using UV-visible (UV-VIS) spectroscopy. Characterization of NPs had been done by X-ray diffraction (XRD) and Fourier transformed infrared spectroscopy (FTIR). Morphology of prepared samples was analyzed by field emission scanning electron microscopy (FESEM) and high resolution transmission electron microscopy (HRTEM) was used to investigate the particle size. Whose results are reported in another article.
Methods: In order to investigate the cytotoxic activity of ZnO nanoparticles synthesized by Green method, the aqueous extract of Hyssopus officinalis plant was used on cells from 3t 3 cell line. To perform the MTT assay the cells were cultured in a flat-bottomed cell culture plates and placed in an incubator for 24 and 48h. After taking the necessary time, the medium was carefully removed and to all the wells were added treatment medium including 7.8 ,15.6 ,31.2, 62.5, 125, and 250 µg/ml of ZnO-NPs/Hf. after 24h, The MTT solution was added to each well and 4 h later the discharge MTT solution and 100 µl of DMSO were added and eventually the absorption was evaluated.
Results: Statistical analysis of the results showed that during incubation period, the percentage of cell viability was reduced by increasing the dose. As a result, the percentage of viability of 3t3 cells was reduced that difference was statistically significant (P≤0.001). The comparison of cytotoxicity levels suggests that the Zn O-NPs inhibits cancerous cells in low concentrations , while not affecting on normal cells.
Conclusion: Conclusion: Zinc oxide nanoparticles biosynthesized with Hyssopus officinalis extract have the ability to induce apoptosis in cancer cells in dose depended manner but no effects on normal cells. Thus this green nanoparticle can be considered as a new promising and effective strategy for the treatment of colon cancer.