3rd International Congress on Biomedicine (ICB2019)
Investigation of zinc oxide synthesized zinc oxide nanoparticles from Hyssopus officinail plant extract on blood parameters and inflammation induced by carrageenan in mice
Investigation of zinc oxide synthesized zinc oxide nanoparticles from Hyssopus officinail plant extract on blood parameters and inflammation induced by carrageenan in mice
Ghasem Rahimi Kalateh Shah Mohammad,1,*vahid pouresmail,2
1. Department of Biology, Mashhad Branch, Islamic Azad University, Mashhad, Iran 2. Assistant Professor, Department of Biochemistry., Faculty of Medicine, Islamic Azad University-Mashhad Branch (IAUM), IRAN
Introduction: Nanotechnology is the science of producing, manipulating and using subatomic materials for the production of new products and processes. Carrageenan, an inflammatory agent, develops and develops acute inflammation in 12 stages in a body, initially mobilizing neutrophils initially in the inflammation of carrageenan in a 20-minute detachable phase. In the production or release of these cells through the walls of the capillaries, certain chemical factors are involved. With the introduction of phagocytosis, lysosomal enzymes released, these enzymes responsible for the activation of the prostaglandin biochemical chain, ultimately with the entry of histamine, serotonin, bradycinin, arachidonic acid, and edema. The purpose of this study was to investigate the effect of ZnO nanoparticles synthesized by Hyssopus officinail plant extract on blood and biochemical parameters of cariogenic inflammation in female rats.
Methods: This research is an experimental study in which 42 white Syrian female rats, weighing about 30 g from the house of animals of the Razi vaccine and serum research institute in Mashhad, were transferred to the Biological Sciences Laboratory of the Faculty of Basic Sciences of Islamic Azad University of Mashhad. Mice were placed in special cages under controlled conditions (temperature of +2.1 ° C and relative humidity of 60 ± 10%, 12 hours of light and 12 hours of darkness) with easy access to water and food in order to adapt to new conditions. They were kept for a week. To prepare the nanoparticles and doses needed, zinc oxide nanoparticles were dissolved with 20 nm in a distilled water (0.3 ml) under ultrasound for 20 minutes. The needed carrageenan was purchased from Sigma Company. Indomethacin 75 mg was purchased as a standard drug from Aria Pharmaceuticals Company and was used to prepare carrageenan and nanoparticles from saline. To prepare indomethacin, after weighing, dried powder was added to a solution of 0.1 mM sodium carbonate and a drop of tin 80 was added to prevent precipitation. All intraperitoneal injections in a volume of 10 ml / kg bodyweight and subcutaneous injection of the foot of the foot, according to a previous study, were performed at a given time interval. Animals were randomly divided into seven groups of six. The non-injected group was considered as the control group. The second group was assigned as a negative control group, which was injected peritoneally 30 min before subcutaneous injection of carrageenan (50 μl), normal saline (0.5 ml). The third group received peritoneal injection 0.5 ml of indomethacin as a positive control group 60 minutes before injection of carrageenan. The fourth group was the only cariogenic injection. In the fifth, sixth and seventh groups 30 minutes before injection of carrageenan, 50, 100 and 150 mg / kg received zinc oxide nanoparticles peritoneum respectively. 24 hours after injection of carrageenan, the mice were sacrificed by ether and blood transfusion was performed from the heart of the mice. In order to prevent clot formation, a blood sample was collected in a test tube containing heparin, then the number of red and white blood cells, platelets, hematocrit, hemoglobin and average volume of hemoglobin, mean hemoglobin, mean hemoglobin concentration and lymphocyte count Monocytes and neutrophils were measured by solenoid concentrations.
Results: Zinc oxide nanoparticles at concentrations of 100 and 150 mg / kg caused increase in white blood cells, hemoglobin and neutrophils, and a significant reduction in red blood cells, hematocrit and lymphocytes at the level of p <0.05.
Conclusion: Our findings indicate that zinc oxide nanoparticles at higher concentrations have more effects on blood and serum parameters. Decreases in parameters such as red blood cells, hematocrit, platelets and lymphocytes, gradually lead to anemia. Cells and cellular organs allow nanoparticles to penetrate these early biological structures and affect their normal functioning, hence the use of nanoparticles in different sectors, especially in the industrial and food sector in the long run with a concentration Higher, the toxicity of nanoparticles and their effects are most noticed. With proper understanding and proper mechanism of nanoparticles and the evaluation of animal and human studies, these nanoparticles can be traced to a proper orientation in clinical and health matters, which require more studies to achieve.