1. Department of Biology, Faculty of Natural Science, University of Tabriz 2. Department of Biology, Faculty of Natural Science, University of Tabriz 3. Department of Biology, Faculty of Natural Science, University of Tabriz
Colorectal cancer is one of the most common cancers in the world. much attention has been given to nutritional supplements that can alter intestinal flora as factors preventing colon cancer. some studies show that fermented products of some strains of lactobacilli could reduce the risks of certain types of cancer and inhibit the growth of certain tumors in vitro assays, animal studies, human studies, epidemiological and intervention studies. during this project, due to the major role of probiotics to control intestinal health, anti-cancer effects of cytoplasmic extract of lactobacilli isolated from traditional dairy products, on hct116 colorectal cancer cell lines examined.
Traditional dairy products samples were collected from the region of azarbayjan and the suspensions were cultured in mrs agar medium. the isolates were identified by biochemical and molecular methods. supernatants of the isolates cultures were collected and their cytotoxicity was evaluated on hct116 cancer cells by mtt assay, fluorescent staining and cell cycle assay.
Cell assays results showed that supernatant of ut1 isolate shows inhibitory of cancer cells proliferation, as well as apoptosis and cell death activity (p<0.05). proper method and time for release of metabolites can vindicate the significant cytotoxic effect of metabolites obtained from isolates in this project compared to other studies. the results showed that the isolate was 99% compatible with lactobacillus casei.
Results achieved in this study suggest that the use of some metabolites of lactobacilli can candidates for studies on compounds having anti-cancer effect.
Lactobacillus, anticancer effects, hct116 cancer cells.