Extensive diversity of caga carboxylic-end motifs in iranian helicobacter pylori strains

Anahita Dah pahlevan,1,* Saeid latifi-navid,2 Saber zahri,3 Seyedeh zahra bakhti,4

1. 1Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, 56199-11367, Iran
2. 1Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, 56199-11367, Iran
3. 1Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, 56199-11367, Iran
4. 1Department of Biology, Faculty of Sciences, University of Mohaghegh Ardabili, Ardabil, 56199-11367, Iran

Abstract

Introduction

The caga protein from different strains of h. pylori has a wide variation in the c-terminal regions, which includes repetitive phosphorylation fractions (epiya motifs). it has been shown that the number and composition of different motifs can be changed based on geographical situations and determine the clinical outcomes. the aim of this study was to determine the diversity and composition of various caga motifs in h. pylori strains obtained from different regions of iran.

Methods

A total of 283 h. pylori strains were obtained from cultures of gastric biopsy and then dna was extracted and genotyped. data were collected and analyzed using spss version 22.

Results

Of the 283 strains, 133 were positive for caga status. frequency of the epiya-a, b, c, and d motifs in iranian patients, was 95/133 (71.4%), 84/133 (63.2%), 92/133 (69.2%), and 81/133 (60.9%), respectively. in our study, the epiya-abcd type of motile was the most frequent compared to the type of epiya-abc (western), epiya-abd (eastern asian), epiya-bcd, epiya-acd, epiya-ab, epiya-ac, epiya-ad, epiya-bc, epiya-bd, and epiya-cd type motifs (38.3%, 10.5%, 1.5%, 2.3%, 4.5%, 3.8%, 5.8%, 3.8%, 1.5%, 3.0%, and 3.8%, respectively).

Conclusion

Therefore, in terms of the diversity of caga carboxylic-end motifs; in iranian strains, epiya-abcd type motif was different compared to those from the eastern asian (epiya-abd) and western (epiya-abc) strains, and iranian motifs may be associated with the severity of gastrointestinal diseases.

Keywords

Helicobacter pylori, caga, epiya motifs, iran.