Synthesis and characterization of hybrid agnps-fgf-1 system for biomedical applications

Mahsa Nemati,1 Tahereh tohidi moghadam,2 Bahram daraei,3 Bijan ranjbar,4,*

1. M.Sc. Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
2. Assistant professor Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
3. Associate professor Department Faculty of Pharmacy, Shaheed Beheshti University of Medical Sciences, Tehran, PO Box 14155-6153, Iran.
4. Professor Department of Biophysics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.

Abstract

Introduction

: designing hybrid systems of nanoparticles and biomolecules with important role in biology and medicine has attracted significant attention in the field of nanobiotechnology. considering the importance of biophysical and biochemical studies in designing nanocarrier systems, study of the interaction between particular biomolecules and nanoparticles of interest is very important. acidic fibroblast growth factor (fgf1) is a biomolecule that plays an important role in cell growth, cell proliferation, cell differentiation, stress response and wound healing process. as the nanocarrier counterpart, agnps have shown remarkable properties such as wound healing and heat-resistance as well as anti-bacterial, -fungi, -inflammatory activity. therefore, biophysical studies on interactions between these nanoparticles and fgf1 can be considered as a critical step before designing hybrid systems with accelerating property in wound healing.

Methods

In this study, agnps were synthesized by chemical reduction method and characterized by uv-visible spectroscopy and dynamic light scattering. complexes of agnps- fgf1 (with various concentrations of the nanoparticles) were analyzed by circular dichroism spectropolarimetry and fluorescence spectroscopy at physiological ph, 37 °c.

Results

As the results showed, the secondary structure of fgf1 altered and its rigidity decreased upon interaction with various concentrations of agnps

Conclusion

. results of this investigation encourage conducting a series of fundamental studies on the structure and function of the bio and nano components of the candidate hybrid structure, prior to the development of any novel nano drug delivery systems.

Keywords

Nanocarrier, acidic fibroblast growth factor, silver nanoparticles, circular dichroism