1. Department of Specialized biology, Science and Research branch, Islamic Azad University, Tehran, Iran 2. Biotechnology Research Center, Venom & Biotherapeutics Molecules Lab., Pasteur Institute of Iran, Tehran, Iran 3. Department of Specialized biology, Science and Research branch, Islamic Azad University, Tehran, Iran
Abstract
Introduction
In the last decade, vegf is considered as an important therapeutic approach in cancer treatment. vegf nano bodies are the new class of antibodies with numerous advantages compared with its older types which enables us to produce smaller and more cost-effective drugs if we can generate peptides derived from these nano bodies with the same functionality. in this study we discuss about designing a vegf nano body-derivative peptide using in silico analysis. it is assumed that the determining region of complement form of nano body is large so that not only it can connect to vegf growth factor but also it can perfectly connect to the nano body.
Methods
A computer analysis was performed for anti-vegf nano bodies and different cdrs using swiss-pdb software in order to predict their third structure. molecular docking was performed for predicted structures using hex software.
Results
: in silico results revealed that cdr3 region of vegf molecule act as a drug-mimicking peptide that can be used instead of complete nano body molecule.
Conclusion
The derivative peptide from vegf molecule can reduce nano body synthesis related costs and can be a suitable substitute for vegf nano body. however, this requires further studies and investigations.
