Evaluating antibacterial activity of copper nanoparticles biosynthesized by aqueous extract of capparis spinosa fruit

Ardalan Maleki chegeni,1 Hossein mahmoudvand,2 Katrin ebrahimi,3,*

1. Student Research Committee, Lorestan University of Medical Sciences, Khorramabad, Iran
2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
3. Department of Biology, Payame Noor University, Tehran, Iran



This study was conducted to synthesize the copper nanoparticles using the aqueous extract of capparis spinosa and also evaluated their antibacterial activities against some pathogenic bacterial strains.


Uv-vis spectroscopy analyses, fourier transform of infrared (ftir), scanning electron microscopy(sem), and energy dispersive x-ray (edx) were used to recognize the synthesized nanoparticles. the methods for evaluating the antibacterial activity of the synthesized copper nanoparticles was using broth microdilution against some gram-positive and gram-negative bacteria and disk diffusion method. after adding the extract to the copper sulfate solution, the color of the solution changed from light blue to yellowish green. existence of a maximum peak at the wavelength of 414 nm confirmed the formation of the copper nanoparticles. ftir spectrum analysis showed that the factor groups created a coating extract on the surface of the nanoparticles.


Scanning electron microscopy demonstrated the size of the particle between 17 and 41 nm. the findings showed that staphylococcus aureus and bacillus cereus as gram-positive bacteria were most susceptible to synthesized copper nanoparticles in comparison with the gram-negative bacteria (klebsiella pneumoniae, and escherichia coli).


The obtained findings demonstrated that the aqueous extract of c.spinosa acts as a reviver and stabilizer factor. the synthesized copper nanoparticles demonstrated activity against both gram-positive and gram-negative bacteria.


Nanoparticles; antimicrobial; green synthesis; copper