2. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran 3. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran 4. Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran
Currently, there is no effective vaccine available, and chemotherapy is the main approach for treatment of cutaneous leishmaniasis (cl). during recent decades, studies have demonstrated that a number of plant-derived compounds may act as new therapeutic tools against leishmaniasis. this study was evaluated the antileishmanial, antioxidant, and cytotoxic activities of quercus infectoria olivier (oak) extract
This extract (0-80 μg/ml) was evaluated in vitro against promastigote and intracellular amastigote forms of leishmania major (mrho/ir/75/er) using mtt assay and in a macro-phage model, respectively. moreover, cytotoxicity effects of oak in murine macrophage cells were tested by mtt assay.
The findings revealed that oak significantly (p<0.05) inhibited the growth rate of promastigote of (ic50 12.65μg/ml) and amastigotes (ic50 10.31μg/ml) as a dose-dependent response. selectivity index of greater than 10 for oak revealed that oak extract had no cytotoxic effects on macrophage cells.
To conclude, the present study showed potent antileishmanial and antioxidant activity of oak extract; whereas this plant had no toxic effect on mammalian cells.
Cutaneous leishmaniasis; leishmania major; macrophage; oak