Detection of pathogenic bacteria in vap patient of loghman hospital by using multiplex pcr technic
Kiadokht Mafi,
1,* Seyed davar siadat ,
2 Farzam vaziri,
3 Mohammadreza hajiesmaeili,
4 Anahita nasrolahzadeh,
5 Nasrin ghaderi rivand,
6
1. Science and Research branch, Islamic Azad University, Tehran, Iran.
2. Pasteur Institute of Iran
3. Pasteur Institute of Iran
4. Loghman Hakim Hospital
5. Science and Research branch, Islamic Azad University, Tehran, Iran.
6. loghman Hakim Hospital
Abstract
Introduction
Ventilator-associated pneumonia(vap) is the most common infection in intensive care unit patients associated with high morbidity rates and elevated economic costs. in the research investigated a rapid detection of the common pathogens in vap by multiple polymerase chain reaction(m-pcr)
Methods
We performed m-pcr for 21 bal samples from intensive care unit of loghman hospital during january 2017 until march 2017. m-pcr were designed to detect , klebsiella pneumonia, staphylococcus aureus and pseudomonas aeruginosa respectively.
Results
Multiplex pcr detected 4 cases of k pneumoniae , 2 cases of s.aureus,1 p. aeruginosa, 2 cases of k. pneumoniae and s. aureus, 3 cases of k.pneumoniae and p. aeruginosa ,0 case of s. aureus and p. aeruginosa. 1 case of k.pneumoniae and p. aeruginosa and s. aureus.
Conclusion
Klebsiella pneumonia was the most frequent detected pathogen. multiplex pcr is rapid test for detecting the etiological agents , of vap.
Keywords
Vap, multiplex pcr ,klebsiella pneumonia, staphylococcus aureus, pseudomonas aeruginosa
