Detection of pathogenic bacteria in vap patient of loghman hospital by using multiplex pcr technic

Kiadokht Mafi,1,* Seyed davar siadat ,2 Farzam vaziri,3 Mohammadreza hajiesmaeili,4 Anahita nasrolahzadeh,5 Nasrin ghaderi rivand,6

1. Science and Research branch, Islamic Azad University, Tehran, Iran.‎
2. Pasteur Institute of Iran
3. Pasteur Institute of Iran
4. Loghman Hakim Hospital
5. Science and Research branch, Islamic Azad University, Tehran, Iran.‎
6. loghman Hakim Hospital

Abstract


Introduction

Ventilator-associated pneumonia(vap) is the most common infection in intensive care unit patients ‎associated with high morbidity rates and elevated economic costs. in the research investigated a rapid ‎detection of the common pathogens in vap by multiple polymerase chain reaction(m-pcr)‎

Methods

We performed m-pcr for 21 bal samples from intensive care unit of loghman hospital during january ‎‎2017 until march 2017. m-pcr were designed to detect , klebsiella pneumonia, staphylococcus aureus and ‎pseudomonas aeruginosa respectively.‎

Results

Multiplex pcr detected 4 cases of k pneumoniae , 2 cases of s.aureus,1 p. aeruginosa, 2 cases of k. ‎pneumoniae and s. aureus, 3 cases of k.pneumoniae and p. aeruginosa ,0 case of s. aureus and p. ‎aeruginosa. 1 case of k.pneumoniae and p. aeruginosa and s. aureus.‎

Conclusion

Klebsiella pneumonia was the most frequent detected pathogen. multiplex pcr is rapid test for detecting ‎the etiological agents , of vap.‎

Keywords

Vap, multiplex pcr ,klebsiella pneumonia, staphylococcus aureus, pseudomonas aeruginosa