A turn-off fluorescent sensor based on carbon dots for sensitive detection of tetracycline in milk

Hamide Ehtesabi,1,* Zeinab bagheri,2

1. Protein Research Center, Shahid Beheshti University
2. Protein Research Center, Shahid Beheshti University



The detection of tetracycline in animal-derived food and drug is very significant, because of its harmful effects on human health even at trace level. any release of tetracycline into the environment can yield bacterial drug resistance. using simple and robust detection procedures are the first step in the control of antibiotic residues in animal-derived food and drug. carbon dots (cds) with one-atom thickness have attracted much attention as a promising type of fluorescence probe nanomaterials and have been wildly used in chemical sensors and bioimaging. herein we developed high sensitive turn-off fluorescent sensor using carbon dots for tetracycline detection.


Blue luminescent carbon dot was fabricated via pyrolysis method and characterized using dynamic light scattering, uv-vis spectrophotometry, transmission electron microscopy and spectrofluorimetry.


For proof of principle, the carbon-dot based sensor was verified in spiked milk samples and the acceptable limit of detection was obtained. the fluorescence intensity of cds in the presence of tetracycline was quenched even in low concentration.


The so–prepared cds can be employed for determining tetracycline residue in animal-derived food and drug


Carbon dot, turn-off sensor, tetracycline