Comparative investingation of effects of the agricultural pesticides (diazinon and chlorpyrifos) on spermatogenesis in male balb/c male mice

Fatemeh Soheili,1,* Parvin torabzadeh,2 Ahmad gerami,3



Diazinon and chlorpyrifos are kind of organophosphate toxins used for agricultural pests. these toxins control acetylcholine enzyme and heart cells and reproductive system. it also cause infertility. because of using a lot of these compounds in rice fields and citrus trees specially in north of iran, we tried to compare effects of diazinon and chlorpyrifos on sex hormones and spermatogenesis trends in balb/c male mice.


Including control, 60 male mice devided into 6 groups (with ten in each) including sham, control and experimental mice (dose of 27.2 , 40.8 mg/kg for chlorpyrifos toxin and dose of 40 and 60 mg/kg for diazinon). experimental groups receive diazinon and chlorpyrifos intraperitoneally for 35 days with two days of rest diluted water inject to sham group. there aren't injection for and blood was taken for test stron evaluation next, epididim extracted for determining motility and number of sperm. they embed on htf medium. testis evaluated for producing sections and studding different cell classes. they evaluated with use of spss software and tukey test and one-way variance analysis.


With use of microscopic evaluation, it is determined that number of spermatozoids cells, spermatids certoli and semmiferous tubules and diameter of seminiferous tubules have significant decrease .also, the number of leydig cells decrease significantly with comparison to control and sham groups. then testostron hormones decrease sperm comparison of experiment and control groups showed that diazinon and chlorpyrifos as environmental factors may affect significantly on sperm and producing sex hormones parameters.


Falling seminiferous tubules which are necessary for producing sperm can increase probability of infertility. in comparison to chlorpyrifos toxins, diazinon has more negative effects. this study suggests more attention to suitable management of using this toxins.


Pesticides, diazinon