1. Department of Laboratory Sciences,Faculty of Paramedicine, Gorgan University of Medical Sciences 2. Department of Laboratory ScienMedical Sciences.ces,Faculty of Paramedicine, Gorgan University of
Abstract
Introduction
The human pathogenic fungus candida albicans is commonly found on mucosal surfaces, such as the vagina. it has been widely proposed that the use of drugs to treat vaginal disorders be combined with nanotechnology because nanosystems of ten potentiate the action of most active constituents, reducing the required dosage and side effects and improving the resulting activity in comparison with conventional treatments.
Methods
Our present work investigated the efficiency of encapsulation of fluconazole in poly (lactic-coglycolic acid) (plga) nanospheres using solid water emulsion solvent evaporation method.
here we examined the effects of low levels of fluconazole and fluconazole-loaded plga with nccls-m27a3 and effect of nanospheres on the ability of 103cell/ml adherence of c. albicans to hela cell by crystal violet staining .
Results
Our studies showed the successful formation of smooth and spherical fluconazole-loaded plga nanospheres, with an encapsulation efficiency of 90.88±0.14% . the particle size distribution showed a range of 56 nm to 80 nm, with the mean particle size being 62 nm. evaluation of these fluconazole-loaded nanospheres was carried out in hela cell lines. results showed cell viability studies revealed that the fluconazole-loaded nanospheres were able to exert a more pronounced effect on the adhesion candida on hela cells as compared to free fluconazole.
Conclusion
Our studies achieved successful proposed that the use of drugs to treat atifungual diseaseie be combined with nanotechnology because nanosystems often potentiate the action of most active constituents, reducing the required dosage and side effects and improving the resulting activity.
